Virus immunocapture provides evidence of CD8 lymphocyte-derived HIV-1 in vivo

Gareth J. Hughes*, Samantha J. Willey, Alexandra Cochrane, Clifford Leen, Jeanne E. Bell, Peter Simmonds

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


OBJECTIVES: To demonstrate that HIV-1 immunocapture with an antibody against CD8 specifically captures virions derived from infected CD8 T cells, and to determine the proportion of HIV-1 derived from CD8 lymphocytes in plasma samples from HIV-infected individuals. METHODS: A virus capture method was developed to enable the detection of HIV-1 virions based upon the presence of certain cell-specific host-derived proteins (CD8, CD3, CD36) within the viral envelope. HIV-1 virions were captured using antibodies against these proteins and levels of bound virus were determined by quantitative reverse transcriptase-polymerase chain reaction. Highly pure CD8 and CD3+CD8- T-cell cultures were used as in-vitro models to determine the specificity of the virus capture technique. RESULTS: The in-vitro model demonstrates that incorporation of the CD8 molecule into released virions is specific to infection of CD8 T cells. Levels of HIV-1 immunocaptured from plasma of infected individuals using the anti-CD8 antibody indicate that up to 15% (range 10-33) of the plasma viral load is derived from CD8 lymphocytes. CONCLUSION: This study demonstrates for the first time that HIV-1-infected CD8 T cells can contribute substantially to levels of circulating virus during the course of infection. Levels of CD8-derived virus did not correlate with the level of infection of circulating CD8 T cells, but do show a significantly good fit to plasma viral loads based on a power model. The extensive infection of CD8 T cells implied by these results may contribute towards immune dysfunction and disease progression to AIDS.

Original languageEnglish
Pages (from-to)1507-1513
Number of pages7
Issue number12
Publication statusPublished - Jul 2007
Externally publishedYes


  • AIDS
  • CD8 cell
  • Disease progression
  • HIV-1
  • Lymphocyte
  • Pathogenesis
  • T-cell


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