Validation of Three Rapid Screening Methods for Detection of Verotoxin-Producing Escherichia coli in Foods: Interlaboratory Study

Katherine L. Capps, Emiline M. Mclaughlin, Alistair W.A. Murray, Clare F. Aldus*, Gary M. Wyatt, Michael W. Peck, Aart Van Amerongen, Renata M.C. Ariëns, Jan H. Wichers, Christopher L. Baylis, David R.A. Wareing, Frederick J. Bolton, Heather Aird, R. Allen, P. Anderson, M. Boughtflower, Q. Chen, A. Davies, J. Dennis, S. J. GibsonR. A. Green, J. Hilton, Frieda Jorgensen, R. Leuschner, C. Loder, B. Mackey, R. Meldrum, I. Millar, T. Reid, A. J. Robinson, I. Robinson, H. Smith, S. Surman, D. Vickers, M. Wood

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    19 Citations (Scopus)

    Abstract

    An Intel-laboratory study was conducted for the validation of 3 methods for the detection of all verotoxin-producing Escherichia coli (VTEC) in foods. The methods were a multi-analyte 1-step lateral flow immunoassay (LFIA) for detection ofE. coli O157 and verotoxin (VT); an enzyme-linked immunosorbent assay targeted against VT1, VT2, and VT2c (VT-ELISA); and a polymerase chain reaction (PCR) method for detection of VT genes (VT-PCR). Aliquots (25 g or 25 mL) of 4 food types (raw minced [ground] beef, unpasteurized milk, unpasteurized apple juice [cider], and salami) were individually inoculated with low numbers (<9 to 375 cells/25 g) of 6 test strains ofE. coli (serogroups O26, O103, O111, O145, and O157) with differing VT-producing capabilities. Five replicates for each test strain and 5 uninoculated samples were prepared for each food type. Fourteen participating laboratories analyzed samples using the LFIA, 9 analyzed the samples by ELISA, and 9 by PCR. The LFIA for O157 and VT had a specificity (correct identification of negative samples) of 92 and 94%, respectively, and a sensitivity (correct identification of positive samples) of 94 and 55%, respectively. The VT-ELISA and VT-PCR had a specificity of 98 and 99%, respectively, and a sensitivity of 89 and 72%, respectively.

    Original languageEnglish
    Pages (from-to)68-77
    Number of pages10
    JournalJournal of AOAC International
    Volume87
    Issue number1
    DOIs
    Publication statusPublished - 2004

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