Validation of a diphtheria toxoid multiplex bead assay for serosurveys

Heather M. Scobie*, Nino Khetsuriani, Androulla Efstratiou, Jeffrey W. Priest

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


We validated a multiplex bead assay for diphtheria toxoid IgG antibodies against the Vero cell toxin neutralization test using 1300 specimens (correlation = 0.88). At the ≥0.01 IU/mL cutoff for minimal seroprotection, sensitivity was 95% and specificity was 83%. Agreement for three categories (<0.01, 0.01–<0.1, ≥0.1 IU/mL) was 81% (kappa = 0.71).

Original languageEnglish
Article number115371
JournalDiagnostic Microbiology and Infectious Disease
Issue number3
Early online date13 Mar 2021
Publication statusE-pub ahead of print - 13 Mar 2021

Bibliographical note

Funding Information:
We would like to thank Kathleen Wannemuehler and Minal Patel at CDC for technical advice on evaluation design. We also thank Paul Stickings at National Institute for Biological Standards and Control, UK; Christina Von Hunolstein at National Institute of Health (ISS), Italy; Jarad Schiffer and Rania Tohme at CDC for helpful discussion about the interpretation of results.

Publisher Copyright:
© 2021


  • Diphtheria
  • Immunity
  • Multiplex assay
  • Serological test
  • Serology
  • Serosurveillance


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