Validation of a diphtheria toxoid multiplex bead assay for serosurveys

Heather M. Scobie; Nino Khetsuriani; Androulla Efstratiou; Jeffrey W. Priest

doi:10.1016/j.diagmicrobio.2021.115371

Validation of a diphtheria toxoid multiplex bead assay for serosurveys

Heather M. Scobie^*, Nino Khetsuriani, Androulla Efstratiou, Jeffrey W. Priest

^*Corresponding author for this work

Colindale RSD Ops

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We validated a multiplex bead assay for diphtheria toxoid IgG antibodies against the Vero cell toxin neutralization test using 1300 specimens (correlation = 0.88). At the ≥0.01 IU/mL cutoff for minimal seroprotection, sensitivity was 95% and specificity was 83%. Agreement for three categories (<0.01, 0.01–<0.1, ≥0.1 IU/mL) was 81% (kappa = 0.71).

Original language	English
Article number	115371
Journal	Diagnostic Microbiology and Infectious Disease
Volume	100
Issue number	3
Early online date	13 Mar 2021
DOIs	https://doi.org/10.1016/j.diagmicrobio.2021.115371
Publication status	E-pub ahead of print - 13 Mar 2021

Bibliographical note

Funding Information:
We would like to thank Kathleen Wannemuehler and Minal Patel at CDC for technical advice on evaluation design. We also thank Paul Stickings at National Institute for Biological Standards and Control, UK; Christina Von Hunolstein at National Institute of Health (ISS), Italy; Jarad Schiffer and Rania Tohme at CDC for helpful discussion about the interpretation of results.

Publisher Copyright:
© 2021

Keywords

Diphtheria
Immunity
Multiplex assay
Serological test
Serology
Serosurveillance

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.diagmicrobio.2021.115371

Cite this

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title = "Validation of a diphtheria toxoid multiplex bead assay for serosurveys",

abstract = "We validated a multiplex bead assay for diphtheria toxoid IgG antibodies against the Vero cell toxin neutralization test using 1300 specimens (correlation = 0.88). At the ≥0.01 IU/mL cutoff for minimal seroprotection, sensitivity was 95% and specificity was 83%. Agreement for three categories (<0.01, 0.01–<0.1, ≥0.1 IU/mL) was 81% (kappa = 0.71).",

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AU - Priest, Jeffrey W.

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KW - Diphtheria

KW - Immunity

KW - Multiplex assay

KW - Serological test

KW - Serology

KW - Serosurveillance

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M3 - Article

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Validation of a diphtheria toxoid multiplex bead assay for serosurveys

Abstract

Bibliographical note

Keywords

UN SDGs

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