Use of the EntericBio Gastro Panel II in a diagnostic microbiology laboratory: challenges and opportunities

Gary McAuliffe; Liselle Bissessor; Deborah Williamson; Sharon Moore; Janet Wilson; Muriel Dufour; Susan Taylor; Arlo Upton

doi:10.1016/j.pathol.2017.02.003

Use of the EntericBio Gastro Panel II in a diagnostic microbiology laboratory: challenges and opportunities

Gary McAuliffe^*
, Liselle Bissessor
, Deborah Williamson
, Sharon Moore
, Janet Wilson
, Muriel Dufour
, Susan Taylor
, Arlo Upton

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

We sought to verify the performance of the EntericBio real-time Gastro Panel 2 (GP2; Serosep, Ireland) assay targeting Salmonella, Shigella, Campylobacter, verotoxin producing E. coli (VTEC), Giardia, and Cryptosporidium in a high-throughput diagnostic laboratory. Following a technical verification period where 237 faecal samples were tested in parallel, guided culture and discrepancy analysis were performed. A total of 13,223 faecal samples were screened by the GP2 assay. During technical verification, sensitivity, specificity, negative and positive predictive value of the GP2 assay compared with conventional methods were 100% (83–100%), 98% (96.7–98.2%), 100% (98.5–100%), and 82.6% (69–83%), respectively. With guided culture, specificity (99.4–100%) and positive predictive value (15–100%) varied by target. Challenges with interpretation of results, particularly for VTEC, Shigella and Salmonella, are discussed.

Original language	English
Pages (from-to)	419-422
Number of pages	4
Journal	Pathology
Volume	49
Issue number	4
DOIs	https://doi.org/10.1016/j.pathol.2017.02.003
Publication status	Published - Jun 2017
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2017 Royal College of Pathologists of Australasia

Keywords

EntericBio
Salmonella
Shigella
faecal PCR
gastroenteritis
verotoxin producing E. coli

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10.1016/j.pathol.2017.02.003

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@article{89e710cd22644eceaa5a67f9fae39748,

title = "Use of the EntericBio Gastro Panel II in a diagnostic microbiology laboratory: challenges and opportunities",

abstract = "We sought to verify the performance of the EntericBio real-time Gastro Panel 2 (GP2; Serosep, Ireland) assay targeting Salmonella, Shigella, Campylobacter, verotoxin producing E. coli (VTEC), Giardia, and Cryptosporidium in a high-throughput diagnostic laboratory. Following a technical verification period where 237 faecal samples were tested in parallel, guided culture and discrepancy analysis were performed. A total of 13,223 faecal samples were screened by the GP2 assay. During technical verification, sensitivity, specificity, negative and positive predictive value of the GP2 assay compared with conventional methods were 100\% (83–100\%), 98\% (96.7–98.2\%), 100\% (98.5–100\%), and 82.6\% (69–83\%), respectively. With guided culture, specificity (99.4–100\%) and positive predictive value (15–100\%) varied by target. Challenges with interpretation of results, particularly for VTEC, Shigella and Salmonella, are discussed.",

keywords = "EntericBio, Salmonella, Shigella, faecal PCR, gastroenteritis, verotoxin producing E. coli",

author = "Gary McAuliffe and Liselle Bissessor and Deborah Williamson and Sharon Moore and Janet Wilson and Muriel Dufour and Susan Taylor and Arlo Upton",

note = "Publisher Copyright: {\textcopyright} 2017 Royal College of Pathologists of Australasia",

year = "2017",

month = jun,

doi = "10.1016/j.pathol.2017.02.003",

language = "English",

volume = "49",

pages = "419--422",

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T1 - Use of the EntericBio Gastro Panel II in a diagnostic microbiology laboratory

T2 - challenges and opportunities

AU - McAuliffe, Gary

AU - Bissessor, Liselle

AU - Williamson, Deborah

AU - Moore, Sharon

AU - Wilson, Janet

AU - Dufour, Muriel

AU - Taylor, Susan

AU - Upton, Arlo

PY - 2017/6

Y1 - 2017/6

N2 - We sought to verify the performance of the EntericBio real-time Gastro Panel 2 (GP2; Serosep, Ireland) assay targeting Salmonella, Shigella, Campylobacter, verotoxin producing E. coli (VTEC), Giardia, and Cryptosporidium in a high-throughput diagnostic laboratory. Following a technical verification period where 237 faecal samples were tested in parallel, guided culture and discrepancy analysis were performed. A total of 13,223 faecal samples were screened by the GP2 assay. During technical verification, sensitivity, specificity, negative and positive predictive value of the GP2 assay compared with conventional methods were 100% (83–100%), 98% (96.7–98.2%), 100% (98.5–100%), and 82.6% (69–83%), respectively. With guided culture, specificity (99.4–100%) and positive predictive value (15–100%) varied by target. Challenges with interpretation of results, particularly for VTEC, Shigella and Salmonella, are discussed.

AB - We sought to verify the performance of the EntericBio real-time Gastro Panel 2 (GP2; Serosep, Ireland) assay targeting Salmonella, Shigella, Campylobacter, verotoxin producing E. coli (VTEC), Giardia, and Cryptosporidium in a high-throughput diagnostic laboratory. Following a technical verification period where 237 faecal samples were tested in parallel, guided culture and discrepancy analysis were performed. A total of 13,223 faecal samples were screened by the GP2 assay. During technical verification, sensitivity, specificity, negative and positive predictive value of the GP2 assay compared with conventional methods were 100% (83–100%), 98% (96.7–98.2%), 100% (98.5–100%), and 82.6% (69–83%), respectively. With guided culture, specificity (99.4–100%) and positive predictive value (15–100%) varied by target. Challenges with interpretation of results, particularly for VTEC, Shigella and Salmonella, are discussed.

KW - EntericBio

KW - Salmonella

KW - Shigella

KW - faecal PCR

KW - gastroenteritis

KW - verotoxin producing E. coli

UR - https://www.scopus.com/pages/publications/85018647608

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DO - 10.1016/j.pathol.2017.02.003

M3 - Article

C2 - 28438393

AN - SCOPUS:85018647608

SN - 0031-3025

VL - 49

SP - 419

EP - 422

JO - Pathology

JF - Pathology

IS - 4

ER -

Use of the EntericBio Gastro Panel II in a diagnostic microbiology laboratory: challenges and opportunities

Abstract

Bibliographical note

Keywords

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