TY - JOUR
T1 - Use of the EntericBio Gastro Panel II in a diagnostic microbiology laboratory
T2 - challenges and opportunities
AU - McAuliffe, Gary
AU - Bissessor, Liselle
AU - Williamson, Deborah
AU - Moore, Sharon
AU - Wilson, Janet
AU - Dufour, Muriel
AU - Taylor, Susan
AU - Upton, Arlo
N1 - Publisher Copyright:
© 2017 Royal College of Pathologists of Australasia
PY - 2017/6
Y1 - 2017/6
N2 - We sought to verify the performance of the EntericBio real-time Gastro Panel 2 (GP2; Serosep, Ireland) assay targeting Salmonella, Shigella, Campylobacter, verotoxin producing E. coli (VTEC), Giardia, and Cryptosporidium in a high-throughput diagnostic laboratory. Following a technical verification period where 237 faecal samples were tested in parallel, guided culture and discrepancy analysis were performed. A total of 13,223 faecal samples were screened by the GP2 assay. During technical verification, sensitivity, specificity, negative and positive predictive value of the GP2 assay compared with conventional methods were 100% (83–100%), 98% (96.7–98.2%), 100% (98.5–100%), and 82.6% (69–83%), respectively. With guided culture, specificity (99.4–100%) and positive predictive value (15–100%) varied by target. Challenges with interpretation of results, particularly for VTEC, Shigella and Salmonella, are discussed.
AB - We sought to verify the performance of the EntericBio real-time Gastro Panel 2 (GP2; Serosep, Ireland) assay targeting Salmonella, Shigella, Campylobacter, verotoxin producing E. coli (VTEC), Giardia, and Cryptosporidium in a high-throughput diagnostic laboratory. Following a technical verification period where 237 faecal samples were tested in parallel, guided culture and discrepancy analysis were performed. A total of 13,223 faecal samples were screened by the GP2 assay. During technical verification, sensitivity, specificity, negative and positive predictive value of the GP2 assay compared with conventional methods were 100% (83–100%), 98% (96.7–98.2%), 100% (98.5–100%), and 82.6% (69–83%), respectively. With guided culture, specificity (99.4–100%) and positive predictive value (15–100%) varied by target. Challenges with interpretation of results, particularly for VTEC, Shigella and Salmonella, are discussed.
KW - EntericBio
KW - faecal PCR
KW - gastroenteritis
KW - Salmonella
KW - Shigella
KW - verotoxin producing E. coli
UR - http://www.scopus.com/inward/record.url?scp=85018647608&partnerID=8YFLogxK
U2 - 10.1016/j.pathol.2017.02.003
DO - 10.1016/j.pathol.2017.02.003
M3 - Article
C2 - 28438393
AN - SCOPUS:85018647608
SN - 0031-3025
VL - 49
SP - 419
EP - 422
JO - Pathology
JF - Pathology
IS - 4
ER -