Use of the EntericBio Gastro Panel II in a diagnostic microbiology laboratory: challenges and opportunities

Gary McAuliffe*, Liselle Bissessor, Deborah Williamson, Sharon Moore, Janet Wilson, Muriel Dufour, Susan Taylor, Arlo Upton

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

We sought to verify the performance of the EntericBio real-time Gastro Panel 2 (GP2; Serosep, Ireland) assay targeting Salmonella, Shigella, Campylobacter, verotoxin producing E. coli (VTEC), Giardia, and Cryptosporidium in a high-throughput diagnostic laboratory. Following a technical verification period where 237 faecal samples were tested in parallel, guided culture and discrepancy analysis were performed. A total of 13,223 faecal samples were screened by the GP2 assay. During technical verification, sensitivity, specificity, negative and positive predictive value of the GP2 assay compared with conventional methods were 100% (83–100%), 98% (96.7–98.2%), 100% (98.5–100%), and 82.6% (69–83%), respectively. With guided culture, specificity (99.4–100%) and positive predictive value (15–100%) varied by target. Challenges with interpretation of results, particularly for VTEC, Shigella and Salmonella, are discussed.

Original languageEnglish
Pages (from-to)419-422
Number of pages4
JournalPathology
Volume49
Issue number4
DOIs
Publication statusPublished - Jun 2017
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2017 Royal College of Pathologists of Australasia

Keywords

  • EntericBio
  • faecal PCR
  • gastroenteritis
  • Salmonella
  • Shigella
  • verotoxin producing E. coli

Fingerprint

Dive into the research topics of 'Use of the EntericBio Gastro Panel II in a diagnostic microbiology laboratory: challenges and opportunities'. Together they form a unique fingerprint.

Cite this