Ultraviolet Radiation-Induced Production of Nitric Oxide:A multi-cell and multi-donor analysis

Graham Holliman*, Donna Lowe, Howard Cohen, Sarah Felton, Ken Raj

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)


Increasing evidence regarding positive effects of exposure to sunlight has led to suggestions that current advice may be overly weighted in favour of avoidance. UV-A has been reported to lower blood pressure, possibly through nitric oxide (NO) production in skin. Here, we set out to investigate effects of UV-A and solar-simulated radiation on the potential source of dermal NO, the effective doses and wavelengths, the responsiveness of different human skin cells, the magnitude of inter-individual differences and the potential influence of age. We utilised isogenic keratinocytes, microvascular endothelial cells, melanocytes and fibroblasts isolated from 36 human skins ranging from neonates to 86 years old. We show that keratinocytes and microvascular endothelial cells show greatest NO release following biologically relevant doses of UV-A. This was consistent across multiple neonatal donors and the effect is maintained in adult keratinocytes. Our observations are consistent with a bi-phasic mechanism by which UV-A can trigger vasodilatory effects. Analyses of NO-production spectra adds further evidence that nitrites in skin cells are the source of UV-mediated NO release. These potentially positive effects of ultraviolet radiation lend support for objective assessment of environmental influence on human health and the idea of "healthy sun exposure".

Original languageEnglish
Article number11105
JournalScientific Reports
Issue number1
Publication statusPublished - 1 Dec 2017

Bibliographical note

Funding Information:
We are grateful to Marina Khazova for her advice and dosimetry of our UV sources, and to Simon Bouffler for his support. The research was funded by the National Institute for Health Research (NIHR) through two Health Protection Research Unit collaborations: Health Impact of Environmental Hazards with King’s College London in partnership with Public Health England (PHE); and Chemical and Radiation Hazards and Threats with Newcastle University in partnership with PHE. The views expressed are those of the author(s) and not necessarily those of the NHS, the NIHR, the Department of Health or Public Health England.

Publisher Copyright:
© 2017 The Author(s).


Dive into the research topics of 'Ultraviolet Radiation-Induced Production of Nitric Oxide:A multi-cell and multi-donor analysis'. Together they form a unique fingerprint.

Cite this