Thiamine Pyrophosphatase from Rabbit Brain

J. R. Cooper

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

This chapter presents the procedure for assay and purification of thiamine pyrophosphatase. Thiamine pyrophosphatase has been purified from liver as well as brain. In both tissues, it appears to have an absolute specificity for thiamine pyrophosphate (TPP) as substrate among the various thiamine phosphate esters, but in addition, it also catalyzes the hydrolysis of certain nucleoside diphosphates. For both the liver and brain enzymes, adenosine triphosphate has an allosteric effect and lowers the Km of the substrate regardless of whether it is a nucleoside diphosphate or TPP.

Original languageEnglish
Pages (from-to)227-229
Number of pages3
JournalMethods in Enzymology
Volume18
DOIs
Publication statusPublished - 1 Jan 1970
Externally publishedYes

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