Thiamine Pyrophosphatase from Rabbit Brain

John Cooper

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)


    This chapter presents the procedure for assay and purification of thiamine pyrophosphatase. Thiamine pyrophosphatase has been purified from liver as well as brain. In both tissues, it appears to have an absolute specificity for thiamine pyrophosphate (TPP) as substrate among the various thiamine phosphate esters, but in addition, it also catalyzes the hydrolysis of certain nucleoside diphosphates. For both the liver and brain enzymes, adenosine triphosphate has an allosteric effect and lowers the Km of the substrate regardless of whether it is a nucleoside diphosphate or TPP.

    Original languageEnglish
    Pages (from-to)227-229
    Number of pages3
    JournalMethods in Enzymology
    Publication statusPublished - 1 Jan 1970


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