The use of germinants to potentiate the sensitivity of Bacillus anthracis spores to peracetic acid

Ozgur Celebi, Fatih Buyuk, Tom Pottage, Ant Crook, Suzanna Hawkey, Callum Cooper, Allan Bennett, Mitat Sahin, Leslie Baillie*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Elimination of Bacillus anthracis spores from the environment is a difficult and costly process due in part to the toxicity of current sporicidal agents. For this reason we investigated the ability of the spore germinants L-alanine (100 mM) and inosine (5 mM) to reduce the concentration of peracetic acid (PAA) required to inactivate B. anthracis spores. While L-alanine significantly enhanced (p = 0.0085) the bactericidal activity of 500 ppm PAA the same was not true for inosine suggesting some form of negative interaction. In contrast the germinant combination proved most effective at 100 ppm PAA (p = 0.0009). To determine if we could achieve similar results in soil we treated soil collected from the burial site of an anthrax infected animal which had been supplemented with spores of the Sterne strain of B. anthracis to increase the level of contamination to 104 spores/g. Treatment with germinants followed 1 h later by 5000 ppm PAA eliminated all of the spores. In contrast direct treatment of the animal burial site using this approach delivered using a back pack sprayer had no detectable effect on the level of B. anthracis contamination or on total culturable bacterial numbers over the course of the experiment. It did trigger a significant, but temporary, reduction (p < 0.0001) in the total spore count suggesting that germination had been triggered under real world conditions. In conclusion, we have shown that the application of germinants increase the sensitivity of bacterial spores to PAA. While the results of the single field trial were inconclusive, the study highlighted the potential of this approach and the challenges faced when attempting to perform real world studies on B. anthracis spores contaminated sites.

Original languageEnglish
Article number00018
JournalFrontiers in Microbiology
Volume7
Issue numberJAN
DOIs
Publication statusPublished - 2016

Bibliographical note

Funding Information:
We would like to acknowledge the financial support of Funda??o para a Ci?ncia e a Tecnologia (FCT, Portugal) through projects PTDC/QEQ-PRS/2824/2012, UID/QUI/50006/2013 (LAQV/REQUIMTE), UID/DTP/04138/2013 (iMed. ULisboa) and PEst-OE/ AGR/UI0245/2014. Carla F. Martins and Andreia Rosatella acknowledge FCT for fellowships SFRH/BD/111128/2015 and SFRH/ BPD/75045/2010, respectively.

Publisher Copyright:
© 2016 Celebi, Buyuk, Pottage, Crook, Hawkey, Cooper, Bennett, Sahin and Baillie.

Keywords

  • Bacillus anthracis
  • Bacterial spores
  • Decontamination
  • Environmental
  • Germination
  • Peracetic acid

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