TY - JOUR
T1 - The second gamma-H2AX assay inter-comparison exercise carried out in the framework of the European biodosimetry network (RENEB)
AU - Moquet, Jayne
AU - Barnard, Stephen
AU - Staynova, Albena
AU - Lindholm, Carita
AU - Monteiro Gil, Octávia
AU - Martins, Vanda
AU - Rößler, Ute
AU - Vral, Anne
AU - Vandevoorde, Charlot
AU - Wojewódzka, Maria
AU - Rothkamm, Kai
N1 - Publisher Copyright:
© 2016 Crown Copyright. Published by Informa UK Limited, trading as Taylor & Francis Group.
PY - 2017/1/2
Y1 - 2017/1/2
N2 - Purpose: Within the EU RENEB project, seven laboratories have taken part in training and harmonisation activities to strengthen triage gamma-H2AX-based radiation exposure assessment. This has culminated in a second triage biodosimetry exercise. Materials and methods: Whole blood and separated lymphocyte samples were homogenously irradiated with 60Co gamma rays at 0.5, 2.5 (blind samples), 0 and 2 Gy (reference samples). Following post-exposure incubations of 4 and 24 h, 16 samples were shipped on ice packs to each partner. The samples were stained and scored for gamma-H2AX foci, using manual and/or automated fluorescence microscope scoring strategies. Dose estimates were obtained and used to assign triage categories to the samples. Results: Average dose estimates across all the laboratories correlated well with true doses. The most accurate assignment of triage category was achieved by manual scoring of the 4-h blood and lymphocyte samples. Only three samples out of a total of 46 were miscategorized in a way that could have adversely effected the clinical management of a radiation casualty. Conclusions: This inter-comparison exercise has demonstrated that following a recent acute radiation exposure, the gamma-H2AX assay could be a useful triage tool that can be successfully applied across a network of laboratories.
AB - Purpose: Within the EU RENEB project, seven laboratories have taken part in training and harmonisation activities to strengthen triage gamma-H2AX-based radiation exposure assessment. This has culminated in a second triage biodosimetry exercise. Materials and methods: Whole blood and separated lymphocyte samples were homogenously irradiated with 60Co gamma rays at 0.5, 2.5 (blind samples), 0 and 2 Gy (reference samples). Following post-exposure incubations of 4 and 24 h, 16 samples were shipped on ice packs to each partner. The samples were stained and scored for gamma-H2AX foci, using manual and/or automated fluorescence microscope scoring strategies. Dose estimates were obtained and used to assign triage categories to the samples. Results: Average dose estimates across all the laboratories correlated well with true doses. The most accurate assignment of triage category was achieved by manual scoring of the 4-h blood and lymphocyte samples. Only three samples out of a total of 46 were miscategorized in a way that could have adversely effected the clinical management of a radiation casualty. Conclusions: This inter-comparison exercise has demonstrated that following a recent acute radiation exposure, the gamma-H2AX assay could be a useful triage tool that can be successfully applied across a network of laboratories.
KW - RENEB
KW - biological dosimetry
KW - gamma-H2AX assay
KW - radiation-induced foci
KW - triage
UR - http://www.scopus.com/inward/record.url?scp=84981731671&partnerID=8YFLogxK
U2 - 10.1080/09553002.2016.1207822
DO - 10.1080/09553002.2016.1207822
M3 - Article
C2 - 27686523
AN - SCOPUS:84981731671
SN - 0955-3002
VL - 93
SP - 58
EP - 64
JO - International Journal of Radiation Biology
JF - International Journal of Radiation Biology
IS - 1
ER -