The Incorporation of [14C]Glucosamine into Dolichol Diphosphate N‐Acetyl[14C]glucosamine by Unbroken Liver Cells in Culture

John Cooper, F. W. HEMMING*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    Incubation of whole Chang liver cells with d‐[1‐14C]glucosamine results in incorporation of radioactivity into both proteins and lipids. A minor (∼ 3%) amount of the labelled lipid has the chromatographic, solubility and chemical properties of dolichol diphosphate N‐acetylglucosamine. A similar compound is formed when membrane preparations of the cells are incubated with UDP‐N‐acetyl[14C]glucosamine. The same membrane fractions catalyse the transfer of [14C]‐mannose from GDP‐[14C]mannose to dolichol phosphate.

    Original languageEnglish
    Pages (from-to)89-94
    Number of pages6
    JournalEuropean Journal of Biochemistry
    Volume78
    Issue number1
    DOIs
    Publication statusPublished - Aug 1977

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