The immunogenicity of outer membrane proteins of haemin‐depleted Porphyromonas (Bacteriodes) Gingvilis W50 in periodontal desease

S. Papaioannou, Phillip Marsh, L. Ivanyi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

The antigens from outer membrane protein extracts of Porphyromonas gingivalis (W50), grown under different haemin concentrations, were examined for binding with serum antibodies from patients with severe progressive periodontitis or from periodontally healthy control subjects. P. gingivalis was grown under haemin limitation (0.33 μg/ml) and haemin excess (2.5 μg/ml) conditions in a chemostat at a mean generation time of 6.9 h, at pH 7.5. Sarkosyl‐insoluble fractions of outer membrane proteins from P. gingivalis were prepared, and analysed by sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and immunoblot techniques. The SDS‐PAGE analysis of the outer membrane of haemin‐limited P. gingivalis identified several new protein components, or changed expression of bands compared with cells grown under haemin excess. Immunoblot analysis showed IgG antibodies to 2 haemin deprivation‐induced proteins in patients with severe progressive periodontitis, but not in the control sera. These results confirm the immunogenicity of some of the haemin‐regulated outer membrane proteins of P. gingivalis in severe progressive periodontitis.

Original languageEnglish
Pages (from-to)327-331
Number of pages5
JournalOral Microbiology and Immunology
Volume6
Issue number6
DOIs
Publication statusPublished - Dec 1991

Keywords

  • antibody
  • haemin
  • periodontal disease
  • Porphyromonas gingivalis

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