TY - JOUR
T1 - The history, genome and biology of NCTC 30
T2 - A non-pandemic Vibrio cholerae isolate from World War One
AU - Dorman, Matthew J.
AU - Kane, Leanne
AU - Domman, Daryl
AU - Turnbull, Jake D.
AU - Cormie, Claire
AU - Fazal, Mohammed Abbas
AU - Goulding, David A.
AU - Russell, Julie E.
AU - Alexander, Sarah
AU - Thomson, Nicholas R.
N1 - Funding Information:
This work was supported by Wellcome (grant no. 206194). M.J.D. is supported by a Wellcome Sanger Institute PhD Studentship.
PY - 2019/4/10
Y1 - 2019/4/10
N2 - The sixth global cholera pandemic lasted from 1899 to 1923. However, despite widespread fear of the disease and of its negative effects on troop morale, very few soldiers in the British Expeditionary Forces contracted cholera between 1914 and 1918. Here, we have revived and sequenced the genome of NCTC 30, a 102-year-old Vibrio cholerae isolate, which we believe is the oldest publicly available live V. cholerae strain in existence. NCTC 30 was isolated in 1916 from a British soldier convalescent in Egypt. We found that this strain does not encode cholera toxin, thought to be necessary to cause cholera, and is not part of V. cholerae lineages responsible for the pandemic disease. We also show that NCTC 30, which predates the introduction of penicillin-based antibiotics, harbours a functional b-lactamase antibiotic resistance gene. Our data corroborate and provide molecular explanations for previous phenotypic studies of NCTC 30 and provide a new high-quality genome sequence for historical, non-pandemic V. cholerae.
AB - The sixth global cholera pandemic lasted from 1899 to 1923. However, despite widespread fear of the disease and of its negative effects on troop morale, very few soldiers in the British Expeditionary Forces contracted cholera between 1914 and 1918. Here, we have revived and sequenced the genome of NCTC 30, a 102-year-old Vibrio cholerae isolate, which we believe is the oldest publicly available live V. cholerae strain in existence. NCTC 30 was isolated in 1916 from a British soldier convalescent in Egypt. We found that this strain does not encode cholera toxin, thought to be necessary to cause cholera, and is not part of V. cholerae lineages responsible for the pandemic disease. We also show that NCTC 30, which predates the introduction of penicillin-based antibiotics, harbours a functional b-lactamase antibiotic resistance gene. Our data corroborate and provide molecular explanations for previous phenotypic studies of NCTC 30 and provide a new high-quality genome sequence for historical, non-pandemic V. cholerae.
KW - Vibrio cholerae
KW - World War One
KW - antimicrobial resistance
KW - cholera
KW - flagella
KW - long-read sequencing
UR - http://www.scopus.com/inward/record.url?scp=85064722185&partnerID=8YFLogxK
U2 - 10.1098/rspb.2018.2025
DO - 10.1098/rspb.2018.2025
M3 - Article
C2 - 30966987
AN - SCOPUS:85064722185
SN - 0962-8452
VL - 286
JO - Proceedings of the Royal Society B: Biological Sciences
JF - Proceedings of the Royal Society B: Biological Sciences
IS - 1900
M1 - 20182025
ER -