The expression in Escherichia coli of sequences coding for the p18 protein of human immunodeficiency virus and the use of the recombinant protein in characterizing a panel of monoclonal antibodies against the viral p18 protein

R. P. Spence; J. Walker; W. M. Jarvill; R. B. Ferns; R. S. Tedder; Q. Sattentau; J. Weber; N. R. Parry; P. E. Highfield

doi:10.1099/0022-1317-70-11-2853

The expression in Escherichia coli of sequences coding for the p18 protein of human immunodeficiency virus and the use of the recombinant protein in characterizing a panel of monoclonal antibodies against the viral p18 protein

R. P. Spence, J. Walker, W. M. Jarvill, R. B. Ferns, R. S. Tedder, Q. Sattentau, J. Weber, N. R. Parry, P. E. Highfield

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9 Citations (Scopus)

Abstract

The sequences coding for the p18 protein of CBL-1, a British human immunodeficiency virus (HIV) type 1 isolate, were expressed in Escherichia coli as β-galactosidase fusion proteins. The recombinant proteins were used to screen a panel of five monoclonal antibodies (MAbs) raised against p18 expressed in CBL-1-infected cells. The regions containing the epitopes for four of the MAbs were located using carboxy deletion mutants and synthetic peptides. The epitope of one of the MAbs (1D9) was reconstructed as part of an unfused, E. coli-expressed p18 protein using the polymerase chain reaction technique. Four different HIV strains and one lymphadenopathy virus type 2 strain were analysed by fluorescence-activated cell sorting of live infected cells using the p18-reactive MAbs.

Original language	English
Pages (from-to)	2853-2863
Number of pages	11
Journal	Journal of General Virology
Volume	70
Issue number	11
DOIs	https://doi.org/10.1099/0022-1317-70-11-2853
Publication status	Published - 1989

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Copyright 2017 Elsevier B.V., All rights reserved.

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Spence, R. P., Walker, J., Jarvill, W. M., Ferns, R. B., Tedder, R. S., Sattentau, Q., Weber, J., Parry, N. R., & Highfield, P. E. (1989). The expression in Escherichia coli of sequences coding for the p18 protein of human immunodeficiency virus and the use of the recombinant protein in characterizing a panel of monoclonal antibodies against the viral p18 protein. Journal of General Virology, 70(11), 2853-2863. https://doi.org/10.1099/0022-1317-70-11-2853

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title = "The expression in Escherichia coli of sequences coding for the p18 protein of human immunodeficiency virus and the use of the recombinant protein in characterizing a panel of monoclonal antibodies against the viral p18 protein",

abstract = "The sequences coding for the p18 protein of CBL-1, a British human immunodeficiency virus (HIV) type 1 isolate, were expressed in Escherichia coli as β-galactosidase fusion proteins. The recombinant proteins were used to screen a panel of five monoclonal antibodies (MAbs) raised against p18 expressed in CBL-1-infected cells. The regions containing the epitopes for four of the MAbs were located using carboxy deletion mutants and synthetic peptides. The epitope of one of the MAbs (1D9) was reconstructed as part of an unfused, E. coli-expressed p18 protein using the polymerase chain reaction technique. Four different HIV strains and one lymphadenopathy virus type 2 strain were analysed by fluorescence-activated cell sorting of live infected cells using the p18-reactive MAbs.",

author = "Spence, \{R. P.\} and J. Walker and Jarvill, \{W. M.\} and Ferns, \{R. B.\} and Tedder, \{R. S.\} and Q. Sattentau and J. Weber and Parry, \{N. R.\} and Highfield, \{P. E.\}",

year = "1989",

doi = "10.1099/0022-1317-70-11-2853",

language = "English",

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Spence, RP, Walker, J, Jarvill, WM, Ferns, RB, Tedder, RS, Sattentau, Q, Weber, J, Parry, NR & Highfield, PE 1989, 'The expression in Escherichia coli of sequences coding for the p18 protein of human immunodeficiency virus and the use of the recombinant protein in characterizing a panel of monoclonal antibodies against the viral p18 protein', Journal of General Virology, vol. 70, no. 11, pp. 2853-2863. https://doi.org/10.1099/0022-1317-70-11-2853

The expression in Escherichia coli of sequences coding for the p18 protein of human immunodeficiency virus and the use of the recombinant protein in characterizing a panel of monoclonal antibodies against the viral p18 protein. / Spence, R. P.; Walker, J.; Jarvill, W. M. et al.
In: Journal of General Virology, Vol. 70, No. 11, 1989, p. 2853-2863.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - The expression in Escherichia coli of sequences coding for the p18 protein of human immunodeficiency virus and the use of the recombinant protein in characterizing a panel of monoclonal antibodies against the viral p18 protein

AU - Spence, R. P.

AU - Walker, J.

AU - Jarvill, W. M.

AU - Ferns, R. B.

AU - Tedder, R. S.

AU - Sattentau, Q.

AU - Weber, J.

AU - Parry, N. R.

AU - Highfield, P. E.

PY - 1989

Y1 - 1989

N2 - The sequences coding for the p18 protein of CBL-1, a British human immunodeficiency virus (HIV) type 1 isolate, were expressed in Escherichia coli as β-galactosidase fusion proteins. The recombinant proteins were used to screen a panel of five monoclonal antibodies (MAbs) raised against p18 expressed in CBL-1-infected cells. The regions containing the epitopes for four of the MAbs were located using carboxy deletion mutants and synthetic peptides. The epitope of one of the MAbs (1D9) was reconstructed as part of an unfused, E. coli-expressed p18 protein using the polymerase chain reaction technique. Four different HIV strains and one lymphadenopathy virus type 2 strain were analysed by fluorescence-activated cell sorting of live infected cells using the p18-reactive MAbs.

AB - The sequences coding for the p18 protein of CBL-1, a British human immunodeficiency virus (HIV) type 1 isolate, were expressed in Escherichia coli as β-galactosidase fusion proteins. The recombinant proteins were used to screen a panel of five monoclonal antibodies (MAbs) raised against p18 expressed in CBL-1-infected cells. The regions containing the epitopes for four of the MAbs were located using carboxy deletion mutants and synthetic peptides. The epitope of one of the MAbs (1D9) was reconstructed as part of an unfused, E. coli-expressed p18 protein using the polymerase chain reaction technique. Four different HIV strains and one lymphadenopathy virus type 2 strain were analysed by fluorescence-activated cell sorting of live infected cells using the p18-reactive MAbs.

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Spence RP, Walker J, Jarvill WM, Ferns RB, Tedder RS, Sattentau Q et al. The expression in Escherichia coli of sequences coding for the p18 protein of human immunodeficiency virus and the use of the recombinant protein in characterizing a panel of monoclonal antibodies against the viral p18 protein. Journal of General Virology. 1989;70(11):2853-2863. doi: 10.1099/0022-1317-70-11-2853

The expression in Escherichia coli of sequences coding for the p18 protein of human immunodeficiency virus and the use of the recombinant protein in characterizing a panel of monoclonal antibodies against the viral p18 protein

Abstract

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