The effect of ionophores on growth and glycosyltransferase production of Streptococcus sanguis

Aileen A. West; C. William Keevil; Philip D. Marsh; Derek C. Ellwood

doi:10.1111/j.1574-6968.1984.tb01442.x

The effect of ionophores on growth and glycosyltransferase production of Streptococcus sanguis

Aileen A. West^*, C. William Keevil, Philip D. Marsh, Derek C. Ellwood

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Batch cultures of Streptococcus sanguis NCTC7865 were grown in complex medium in the presence and absence of the ionophores gramicidin, valinomycin and nigericin, to study their effects on growth and glycosyltransferase production. Growth of S. sanguis was markedly inhibited by nigericin or gramicidin, whereas valinomycin had no significant effect. The presence of ionophores caused only slight decreases in glucosyltransferase activity. Fructosyltransferase activity was however reduced by at least 90%. The results indicate that ΔpH rather than ΔΨ is essential for maintaining normal growth in S. sanguis. However, both ΔpH and ΔΨ are necessary for fructosyltransferase synthesis and secretion, but are not apparently involved in the synthesis and secretion of glucosyltransferase.

Original language	English
Pages (from-to)	133-137
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	25
Issue number	2-3
DOIs	https://doi.org/10.1111/j.1574-6968.1984.tb01442.x
Publication status	Published - Dec 1984

Bibliographical note

Funding Information:
This work was funded by a grant from the Medical Research Council of Great Britain (G8310210SB).

Keywords

Fructosyltransferase
Streptococci
protonmotive force

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10.1111/j.1574-6968.1984.tb01442.x

Cite this

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title = "The effect of ionophores on growth and glycosyltransferase production of Streptococcus sanguis",

abstract = "Batch cultures of Streptococcus sanguis NCTC7865 were grown in complex medium in the presence and absence of the ionophores gramicidin, valinomycin and nigericin, to study their effects on growth and glycosyltransferase production. Growth of S. sanguis was markedly inhibited by nigericin or gramicidin, whereas valinomycin had no significant effect. The presence of ionophores caused only slight decreases in glucosyltransferase activity. Fructosyltransferase activity was however reduced by at least 90%. The results indicate that ΔpH rather than ΔΨ is essential for maintaining normal growth in S. sanguis. However, both ΔpH and ΔΨ are necessary for fructosyltransferase synthesis and secretion, but are not apparently involved in the synthesis and secretion of glucosyltransferase.",

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author = "West, {Aileen A.} and Keevil, {C. William} and Marsh, {Philip D.} and Ellwood, {Derek C.}",

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AU - West, Aileen A.

AU - Keevil, C. William

AU - Marsh, Philip D.

AU - Ellwood, Derek C.

N1 - Funding Information: This work was funded by a grant from the Medical Research Council of Great Britain (G8310210SB).

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AB - Batch cultures of Streptococcus sanguis NCTC7865 were grown in complex medium in the presence and absence of the ionophores gramicidin, valinomycin and nigericin, to study their effects on growth and glycosyltransferase production. Growth of S. sanguis was markedly inhibited by nigericin or gramicidin, whereas valinomycin had no significant effect. The presence of ionophores caused only slight decreases in glucosyltransferase activity. Fructosyltransferase activity was however reduced by at least 90%. The results indicate that ΔpH rather than ΔΨ is essential for maintaining normal growth in S. sanguis. However, both ΔpH and ΔΨ are necessary for fructosyltransferase synthesis and secretion, but are not apparently involved in the synthesis and secretion of glucosyltransferase.

KW - Fructosyltransferase

KW - Streptococci

KW - protonmotive force

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The effect of ionophores on growth and glycosyltransferase production of Streptococcus sanguis

Abstract

Bibliographical note

Keywords

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