The development of a novel diagnostic assay that utilizes a pseudotyped vesicular stomatitis virus for the detection of neutralizing activity against crimean-congo hemorrhagic fever virus

Yuto Suda, John Chamberlain, Stuart Dowall, Masayuki Saijo, Taisuke Horimoto, Roger Hewson, Masayuki Shimojima*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Crimean-Congo hemorrhagic fever virus is a risk group 4 pathogen, which mandates the use of maximum containment facilities, often termed biosafety level 4 or containment level 4 when working with infectious materials. Diagnostic and research work involving live viruses in such laboratories is timeconsuming and inconvenient, resulting in delays. Herein, we show that serum neutralizing activity against the virus can be measured in low-containment laboratories using a pseudotyped virus.

Original languageEnglish
Pages (from-to)205-208
Number of pages4
JournalJapanese Journal of Infectious Diseases
Volume71
Issue number3
DOIs
Publication statusPublished - 2018

Bibliographical note

Funding Information:
Acknowledgments We thank Ms. Momoko Ogata of the National Institute of Infectious Diseases, Japan, for her technical assistance. This work was supported in part by grant for Research on Emerging and Re-emerging Infectious Diseases from the Ministry of Health, Labour and Welfare of Japan (H25-Shinko-Ippan-004), by the Japan Society for the Promotion of Science KAKENHI (Grant Numbers 16K08041 and 25670222), and by the Research Program on Emerging and Re-emerging Infectious Diseases from the Japan Agency for Medical Research and Development (AMED).

Publisher Copyright:
© 2018, National Institute of Health. All rights reserved.

Keywords

  • Biosafety level
  • Crimean-Congo hemorrhagic fever virus
  • Pseudotyped virus

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