The antimicrobial activity of prototype modified honeys that generate reactive oxygen species (ROS) hydrogen peroxide

Jonathan Cooke*, Matthew Dryden, Thomas Patton, James Brennan, John Barrett

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

68 Citations (Scopus)


Background: Antimicrobial resistance continues to be a global issue in healthcare organisations. Honey has long been shown to possess wound healing and antimicrobial properties that are dependent on a number of physical and chemical properties of the honey. We tested the antimicrobial activity of a medicinal honey, Surgihoney® (SH) and two prototype modified honeys made by Apis mellifera (honeybee) against Staphylococcus aureus (NCIMB 9518). We also examined the modified honey prototypes for the ability to generate reactive oxygen species (ROS) by changing the level of production of hydrogen peroxide from the samples. Methods: Surgihoney® (SH) was compared with two modified honeys, Prototype 1 (PT1) and Prototype 2 (PT2) using a bioassay method against a standard strain of Staphylococcus aureus. Further work studied the rate of generation of ROS hydrogen peroxide from these preparations. Results: Surgihoney® antimicrobial activity was shown to be largely due to ROS hydrogen peroxide production. By modification of Surgihoney®, two more potent honey prototypes were shown to generate between a two- and three-fold greater antibacterial activity and up to ten times greater ROS peroxide activity. Conclusions: Surgihoney® is a clinically available wound antiseptic dressing that shows good antimicrobial activity. Two further honey prototypes have been shown to have antimicrobial activity that is possible to be enhanced due to demonstrated increases in ROS peroxide activity.

Original languageEnglish
Article number20
JournalBMC Research Notes
Issue number1
Publication statusPublished - 14 Dec 2015

Bibliographical note

Publisher Copyright:
© 2015 Cooke et al.; licensee BioMed Central.


  • Antimicrobial
  • Honey
  • Hydrogen peroxide
  • Modified honey
  • Reactive oxygen species (ROS)
  • Wound dressings


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