The 5th International Conference on Basic and Therapeutic Aspects of Botulinum and Tetanus Neurotoxins. Workshop review: Assays and detection

Clifford Shone*, Joseph Ferreira, Anne Boyer, Nick Cirino, Christina Egan, Elizabeth Evans, John Kools, Shashi Sharma

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    16 Citations (Scopus)

    Abstract

    The development of diagnostic tests for the botulinum neurotoxins is complicated by their extremely high potencies and the considerable diversity observed within the neurotoxin family. Current approaches for the detection of the toxins and the organism include amplified immunoassays and PCR techniques. Assays which exploit the biological activities within the botulinum toxins are also in development. These are based on both antibody and mass spectrometric techniques which measure the endopeptidase activities of the neurotoxins. This overview of the Assays and Detection Workshop of the 5th International Conference on Basic and Therapeutic Aspects of Botulinum and Tetanus Neurotoxins discusses recent progress in the development of these assay systems and the issues that need to be overcome prior to their implementation.

    Original languageEnglish
    Pages (from-to)205-216
    Number of pages12
    JournalNeurotoxicity Research
    Volume9
    Issue number2-3
    DOIs
    Publication statusPublished - 2006

    Bibliographical note

    Funding Information:
    Work at the Health Protection Agency is supported by the UK Department of Health and a grant to C.S. from the National Institute of Allergy and Infectious Diseases (RO1 AI055578). C.E. would like to acknowledge Stephen Davis and George Hannett from the Wadsworth Center, NYSDOH, for their contributions to this work. A.B. thanks John Barr, Hercules Moura, Suzy Kalb, Adrian Woolfitt, Lisa McWilliams, and Jurgen Schmidt who have made major contributions to the development of the Endopep-MS method.

    Keywords

    • Assay
    • Botulinum
    • Detection
    • Endopeptidase
    • Neurotoxin
    • PCR

    Fingerprint

    Dive into the research topics of 'The 5th International Conference on Basic and Therapeutic Aspects of Botulinum and Tetanus Neurotoxins. Workshop review: Assays and detection'. Together they form a unique fingerprint.

    Cite this