Tandem Mass Spectrometry Analysis as an Approach to Delineate Genetically Related Taxa

Raju V. Misra*, Tom Gaulton, Nadia Ahmod, Min Fang, Martin Hornshaw, Jenny Ho, Saheer Gharbia, Haroun N. Shah

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


This chapter demonstrates through worked examples the clinical utility of tandem mass spectrometry (MS). It uses a model of the challenging family Enterobacteriaceae, ultimately showing how proteomics, combined with genomics, can be used to identify and characterize a recent outbreak strain of Escherichia coli, strain type 0104:H4. The chapter also demonstrates the utility of proteomics as a biomarker discovery and validation platform for the identification of protein and genomic features, for the delineation of high-profile, highly pathogenic biothreat agents. In the chapter, a robust method is established and reported the first matrix-assisted laser desorption/ionization-time-of-flight MS (MALDI-TOF MS) microbial database using type and reference strains held in the National Collection of Type Collections. MALDI-TOF MS has ushered in a new era in clinical diagnostics, resulting in greatly reduced costs and rapid species identification. It is now routinely used for the identification of species, profiling mainly ribosomal proteins, but generally excludes expressed virulence factors.

Original languageEnglish
Title of host publicationMALDI-TOF and Tandem MS for Clinical Microbiology
Number of pages67
ISBN (Electronic)9781118960226
ISBN (Print)9781118960257
Publication statusPublished - 15 Sep 2016


  • Candidate biomarker discovery
  • Clinical diagnostics
  • Clinical microbiology
  • E. coli
  • Enterobacteriaceae
  • Pathogenic biothreat agents
  • Strain panel
  • Tandem mass spectrometry


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