Surveillance of human rotaviruses in 2007-2011, Hungary: Exploring the genetic relatedness between vaccine and field strains

Brigitta László; József Kónya; Eszter Dandár; Judit Deák; Ágnes Farkas; James Gray; Gábor Grósz; Miren Iturriza-Go'Mara; Ferenc Jakab; Ágnes Juhász; Péter Kisfali; Julianna Kovács; György Lengyel; Vito Martella; Béla Melegh; Júlia Mészáros; Péter Molnár; Zoltán Nyúl; Hajnalka Papp; László Pátri; Erzsébet Puskás; Ildikó Sántha; Ferenc Schneider; Katalin Szomor; András Tóth; Erzsébet Tóth; György Szuács; Krisztián Bányai

doi:10.1016/j.jcv.2012.06.016

Surveillance of human rotaviruses in 2007-2011, Hungary: Exploring the genetic relatedness between vaccine and field strains

Brigitta László^*
, József Kónya
, Eszter Dandár
, Judit Deák
, Ágnes Farkas
, James Gray
, Gábor Grósz
, Miren Iturriza-Go'Mara
, Ferenc Jakab
, Ágnes Juhász
, Péter Kisfali
, Julianna Kovács
, György Lengyel
, Vito Martella
, Béla Melegh
, Júlia Mészáros
, Péter Molnár
, Zoltán Nyúl
, Hajnalka Papp
, László Pátri

Erzsébet Puskás, Ildikó Sántha, Ferenc Schneider, Katalin Szomor, András Tóth, Erzsébet Tóth, György Szuács, Krisztián Bányai

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Background: The availability of rotavirus vaccines has resulted in an intensification of post vaccine strain surveillance efforts worldwide to gain information on the impact of vaccines on prevalence of circulating rotavirus strains. Objectives: In this study, the distribution of human rotavirus G and P types in Hungary is reported. In addition, the VP4 and VP7 genes of G1P[8] strains were sequenced to monitor if vaccine-derived strains were introduced and/or some strains/lineages were selected against. Study design: The study was conducted in 8 geographic areas of Hungary between 2007 and 2011. Rotavirus positive stool samples were collected from diarrheic patients mostly <5. years of age. Viral RNA was amplified by multiplex genotyping RT-PCR assay, targeting the medically most important G and P types. When needed, sequencing of the VP7 and VP4 genes was performed. Results: In total, 2380 strains were genotyped. During the 5-year surveillance we observed the dominating prevalence of genotype G1P[8] (44.87%) strains, followed by G4P[8] (23.4%), G2P[4] (14.75%) and G9P[8] (6.81%) genotypes. Uncommon strains were identified in a low percentage of samples (4.12%). Phylogenetic analysis of 318 G1P[8] strains identified 55 strains similar to the Rotarix strain (nt sequence identities; VP7, up to 97.9%; VP4, up to 98.5%) although their vaccine origin was unlikely. Conclusions: Current vaccines would have protected against the majority of identified rotavirus genotypes. A better understanding of the potential long-term effect of vaccine use on epidemiology and evolutionary dynamics of co-circulating wild type strains requires continuous strain surveillance.

Original language	English
Pages (from-to)	140-146
Number of pages	7
Journal	Journal of Clinical Virology
Volume	55
Issue number	2
DOIs	https://doi.org/10.1016/j.jcv.2012.06.016
Publication status	Published - Oct 2012

Bibliographical note

Funding Information:
Funding was provided, in equal parts, by Glaxo-SmithKline Biologicals and Sanofi Pasteur MSD in the form of unrestricted collaborative grants and administered through the Health Protection Agency (London, UK). Additional funding was given by the Hungarian Scientific Research Fund (OTKA, K 100727). B. L.’s Ph.D. stipend was given by the Sanofi-Aventis Hungary and then was supported by the Hungarian Academy of Sciences . K.B. was supported by the Momentum Initiative .

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Gastroenteritis
Genotype
Molecular epidemiology
VP4
VP7

Access to Document

10.1016/j.jcv.2012.06.016

Cite this

László, B., Kónya, J., Dandár, E., Deák, J., Farkas, Á., Gray, J., Grósz, G., Iturriza-Go'Mara, M., Jakab, F., Juhász, Á., Kisfali, P., Kovács, J., Lengyel, G., Martella, V., Melegh, B., Mészáros, J., Molnár, P., Nyúl, Z., Papp, H., ... Bányai, K. (2012). Surveillance of human rotaviruses in 2007-2011, Hungary: Exploring the genetic relatedness between vaccine and field strains. Journal of Clinical Virology, 55(2), 140-146. https://doi.org/10.1016/j.jcv.2012.06.016

@article{72c8cc3428344ec896f183919e7e5534,

title = "Surveillance of human rotaviruses in 2007-2011, Hungary: Exploring the genetic relatedness between vaccine and field strains",

abstract = "Background: The availability of rotavirus vaccines has resulted in an intensification of post vaccine strain surveillance efforts worldwide to gain information on the impact of vaccines on prevalence of circulating rotavirus strains. Objectives: In this study, the distribution of human rotavirus G and P types in Hungary is reported. In addition, the VP4 and VP7 genes of G1P[8] strains were sequenced to monitor if vaccine-derived strains were introduced and/or some strains/lineages were selected against. Study design: The study was conducted in 8 geographic areas of Hungary between 2007 and 2011. Rotavirus positive stool samples were collected from diarrheic patients mostly <5. years of age. Viral RNA was amplified by multiplex genotyping RT-PCR assay, targeting the medically most important G and P types. When needed, sequencing of the VP7 and VP4 genes was performed. Results: In total, 2380 strains were genotyped. During the 5-year surveillance we observed the dominating prevalence of genotype G1P[8] (44.87\%) strains, followed by G4P[8] (23.4\%), G2P[4] (14.75\%) and G9P[8] (6.81\%) genotypes. Uncommon strains were identified in a low percentage of samples (4.12\%). Phylogenetic analysis of 318 G1P[8] strains identified 55 strains similar to the Rotarix strain (nt sequence identities; VP7, up to 97.9\%; VP4, up to 98.5\%) although their vaccine origin was unlikely. Conclusions: Current vaccines would have protected against the majority of identified rotavirus genotypes. A better understanding of the potential long-term effect of vaccine use on epidemiology and evolutionary dynamics of co-circulating wild type strains requires continuous strain surveillance.",

keywords = "Gastroenteritis, Genotype, Molecular epidemiology, VP4, VP7",

author = "Brigitta L{\'a}szl{\'o} and J{\'o}zsef K{\'o}nya and Eszter Dand{\'a}r and Judit De{\'a}k and {\'A}gnes Farkas and James Gray and G{\'a}bor Gr{\'o}sz and Miren Iturriza-Go'Mara and Ferenc Jakab and {\'A}gnes Juh{\'a}sz and P{\'e}ter Kisfali and Julianna Kov{\'a}cs and Gy{\"o}rgy Lengyel and Vito Martella and B{\'e}la Melegh and J{\'u}lia M{\'e}sz{\'a}ros and P{\'e}ter Moln{\'a}r and Zolt{\'a}n Ny{\'u}l and Hajnalka Papp and L{\'a}szl{\'o} P{\'a}tri and Erzs{\'e}bet Pusk{\'a}s and Ildik{\'o} S{\'a}ntha and Ferenc Schneider and Katalin Szomor and Andr{\'a}s T{\'o}th and Erzs{\'e}bet T{\'o}th and Gy{\"o}rgy Szu{\'a}cs and Kriszti{\'a}n B{\'a}nyai",

note = "Funding Information: Funding was provided, in equal parts, by Glaxo-SmithKline Biologicals and Sanofi Pasteur MSD in the form of unrestricted collaborative grants and administered through the Health Protection Agency (London, UK). Additional funding was given by the Hungarian Scientific Research Fund (OTKA, K 100727). B. L.{\textquoteright}s Ph.D. stipend was given by the Sanofi-Aventis Hungary and then was supported by the Hungarian Academy of Sciences . K.B. was supported by the Momentum Initiative . ",

year = "2012",

month = oct,

doi = "10.1016/j.jcv.2012.06.016",

language = "English",

volume = "55",

pages = "140--146",

journal = "Journal of Clinical Virology",

issn = "1386-6532",

publisher = "Elsevier B.V.",

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László, B, Kónya, J, Dandár, E, Deák, J, Farkas, Á, Gray, J, Grósz, G, Iturriza-Go'Mara, M, Jakab, F, Juhász, Á, Kisfali, P, Kovács, J, Lengyel, G, Martella, V, Melegh, B, Mészáros, J, Molnár, P, Nyúl, Z, Papp, H, Pátri, L, Puskás, E, Sántha, I, Schneider, F, Szomor, K, Tóth, A, Tóth, E, Szuács, G & Bányai, K 2012, 'Surveillance of human rotaviruses in 2007-2011, Hungary: Exploring the genetic relatedness between vaccine and field strains', Journal of Clinical Virology, vol. 55, no. 2, pp. 140-146. https://doi.org/10.1016/j.jcv.2012.06.016

TY - JOUR

T1 - Surveillance of human rotaviruses in 2007-2011, Hungary

T2 - Exploring the genetic relatedness between vaccine and field strains

AU - László, Brigitta

AU - Kónya, József

AU - Dandár, Eszter

AU - Deák, Judit

AU - Farkas, Ágnes

AU - Gray, James

AU - Grósz, Gábor

AU - Iturriza-Go'Mara, Miren

AU - Jakab, Ferenc

AU - Juhász, Ágnes

AU - Kisfali, Péter

AU - Kovács, Julianna

AU - Lengyel, György

AU - Martella, Vito

AU - Melegh, Béla

AU - Mészáros, Júlia

AU - Molnár, Péter

AU - Nyúl, Zoltán

AU - Papp, Hajnalka

AU - Pátri, László

AU - Puskás, Erzsébet

AU - Sántha, Ildikó

AU - Schneider, Ferenc

AU - Szomor, Katalin

AU - Tóth, András

AU - Tóth, Erzsébet

AU - Szuács, György

AU - Bányai, Krisztián

N1 - Funding Information: Funding was provided, in equal parts, by Glaxo-SmithKline Biologicals and Sanofi Pasteur MSD in the form of unrestricted collaborative grants and administered through the Health Protection Agency (London, UK). Additional funding was given by the Hungarian Scientific Research Fund (OTKA, K 100727). B. L.’s Ph.D. stipend was given by the Sanofi-Aventis Hungary and then was supported by the Hungarian Academy of Sciences . K.B. was supported by the Momentum Initiative .

PY - 2012/10

Y1 - 2012/10

N2 - Background: The availability of rotavirus vaccines has resulted in an intensification of post vaccine strain surveillance efforts worldwide to gain information on the impact of vaccines on prevalence of circulating rotavirus strains. Objectives: In this study, the distribution of human rotavirus G and P types in Hungary is reported. In addition, the VP4 and VP7 genes of G1P[8] strains were sequenced to monitor if vaccine-derived strains were introduced and/or some strains/lineages were selected against. Study design: The study was conducted in 8 geographic areas of Hungary between 2007 and 2011. Rotavirus positive stool samples were collected from diarrheic patients mostly <5. years of age. Viral RNA was amplified by multiplex genotyping RT-PCR assay, targeting the medically most important G and P types. When needed, sequencing of the VP7 and VP4 genes was performed. Results: In total, 2380 strains were genotyped. During the 5-year surveillance we observed the dominating prevalence of genotype G1P[8] (44.87%) strains, followed by G4P[8] (23.4%), G2P[4] (14.75%) and G9P[8] (6.81%) genotypes. Uncommon strains were identified in a low percentage of samples (4.12%). Phylogenetic analysis of 318 G1P[8] strains identified 55 strains similar to the Rotarix strain (nt sequence identities; VP7, up to 97.9%; VP4, up to 98.5%) although their vaccine origin was unlikely. Conclusions: Current vaccines would have protected against the majority of identified rotavirus genotypes. A better understanding of the potential long-term effect of vaccine use on epidemiology and evolutionary dynamics of co-circulating wild type strains requires continuous strain surveillance.

AB - Background: The availability of rotavirus vaccines has resulted in an intensification of post vaccine strain surveillance efforts worldwide to gain information on the impact of vaccines on prevalence of circulating rotavirus strains. Objectives: In this study, the distribution of human rotavirus G and P types in Hungary is reported. In addition, the VP4 and VP7 genes of G1P[8] strains were sequenced to monitor if vaccine-derived strains were introduced and/or some strains/lineages were selected against. Study design: The study was conducted in 8 geographic areas of Hungary between 2007 and 2011. Rotavirus positive stool samples were collected from diarrheic patients mostly <5. years of age. Viral RNA was amplified by multiplex genotyping RT-PCR assay, targeting the medically most important G and P types. When needed, sequencing of the VP7 and VP4 genes was performed. Results: In total, 2380 strains were genotyped. During the 5-year surveillance we observed the dominating prevalence of genotype G1P[8] (44.87%) strains, followed by G4P[8] (23.4%), G2P[4] (14.75%) and G9P[8] (6.81%) genotypes. Uncommon strains were identified in a low percentage of samples (4.12%). Phylogenetic analysis of 318 G1P[8] strains identified 55 strains similar to the Rotarix strain (nt sequence identities; VP7, up to 97.9%; VP4, up to 98.5%) although their vaccine origin was unlikely. Conclusions: Current vaccines would have protected against the majority of identified rotavirus genotypes. A better understanding of the potential long-term effect of vaccine use on epidemiology and evolutionary dynamics of co-circulating wild type strains requires continuous strain surveillance.

KW - Gastroenteritis

KW - Genotype

KW - Molecular epidemiology

KW - VP4

KW - VP7

UR - https://www.scopus.com/pages/publications/84865185482

U2 - 10.1016/j.jcv.2012.06.016

DO - 10.1016/j.jcv.2012.06.016

M3 - Article

C2 - 22841750

AN - SCOPUS:84865185482

SN - 1386-6532

VL - 55

SP - 140

EP - 146

JO - Journal of Clinical Virology

JF - Journal of Clinical Virology

IS - 2

ER -

Surveillance of human rotaviruses in 2007-2011, Hungary: Exploring the genetic relatedness between vaccine and field strains

Abstract

Bibliographical note

UN SDGs

Keywords

Access to Document

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