TY - JOUR
T1 - Surveillance of human rotaviruses in 2007-2011, Hungary
T2 - Exploring the genetic relatedness between vaccine and field strains
AU - László, Brigitta
AU - Kónya, József
AU - Dandár, Eszter
AU - Deák, Judit
AU - Farkas, Ágnes
AU - Gray, James
AU - Grósz, Gábor
AU - Iturriza-Go'Mara, Miren
AU - Jakab, Ferenc
AU - Juhász, Ágnes
AU - Kisfali, Péter
AU - Kovács, Julianna
AU - Lengyel, György
AU - Martella, Vito
AU - Melegh, Béla
AU - Mészáros, Júlia
AU - Molnár, Péter
AU - Nyúl, Zoltán
AU - Papp, Hajnalka
AU - Pátri, László
AU - Puskás, Erzsébet
AU - Sántha, Ildikó
AU - Schneider, Ferenc
AU - Szomor, Katalin
AU - Tóth, András
AU - Tóth, Erzsébet
AU - Szuács, György
AU - Bányai, Krisztián
N1 - Funding Information:
Funding was provided, in equal parts, by Glaxo-SmithKline Biologicals and Sanofi Pasteur MSD in the form of unrestricted collaborative grants and administered through the Health Protection Agency (London, UK). Additional funding was given by the Hungarian Scientific Research Fund (OTKA, K 100727). B. L.’s Ph.D. stipend was given by the Sanofi-Aventis Hungary and then was supported by the Hungarian Academy of Sciences . K.B. was supported by the Momentum Initiative .
PY - 2012/10
Y1 - 2012/10
N2 - Background: The availability of rotavirus vaccines has resulted in an intensification of post vaccine strain surveillance efforts worldwide to gain information on the impact of vaccines on prevalence of circulating rotavirus strains. Objectives: In this study, the distribution of human rotavirus G and P types in Hungary is reported. In addition, the VP4 and VP7 genes of G1P[8] strains were sequenced to monitor if vaccine-derived strains were introduced and/or some strains/lineages were selected against. Study design: The study was conducted in 8 geographic areas of Hungary between 2007 and 2011. Rotavirus positive stool samples were collected from diarrheic patients mostly <5. years of age. Viral RNA was amplified by multiplex genotyping RT-PCR assay, targeting the medically most important G and P types. When needed, sequencing of the VP7 and VP4 genes was performed. Results: In total, 2380 strains were genotyped. During the 5-year surveillance we observed the dominating prevalence of genotype G1P[8] (44.87%) strains, followed by G4P[8] (23.4%), G2P[4] (14.75%) and G9P[8] (6.81%) genotypes. Uncommon strains were identified in a low percentage of samples (4.12%). Phylogenetic analysis of 318 G1P[8] strains identified 55 strains similar to the Rotarix strain (nt sequence identities; VP7, up to 97.9%; VP4, up to 98.5%) although their vaccine origin was unlikely. Conclusions: Current vaccines would have protected against the majority of identified rotavirus genotypes. A better understanding of the potential long-term effect of vaccine use on epidemiology and evolutionary dynamics of co-circulating wild type strains requires continuous strain surveillance.
AB - Background: The availability of rotavirus vaccines has resulted in an intensification of post vaccine strain surveillance efforts worldwide to gain information on the impact of vaccines on prevalence of circulating rotavirus strains. Objectives: In this study, the distribution of human rotavirus G and P types in Hungary is reported. In addition, the VP4 and VP7 genes of G1P[8] strains were sequenced to monitor if vaccine-derived strains were introduced and/or some strains/lineages were selected against. Study design: The study was conducted in 8 geographic areas of Hungary between 2007 and 2011. Rotavirus positive stool samples were collected from diarrheic patients mostly <5. years of age. Viral RNA was amplified by multiplex genotyping RT-PCR assay, targeting the medically most important G and P types. When needed, sequencing of the VP7 and VP4 genes was performed. Results: In total, 2380 strains were genotyped. During the 5-year surveillance we observed the dominating prevalence of genotype G1P[8] (44.87%) strains, followed by G4P[8] (23.4%), G2P[4] (14.75%) and G9P[8] (6.81%) genotypes. Uncommon strains were identified in a low percentage of samples (4.12%). Phylogenetic analysis of 318 G1P[8] strains identified 55 strains similar to the Rotarix strain (nt sequence identities; VP7, up to 97.9%; VP4, up to 98.5%) although their vaccine origin was unlikely. Conclusions: Current vaccines would have protected against the majority of identified rotavirus genotypes. A better understanding of the potential long-term effect of vaccine use on epidemiology and evolutionary dynamics of co-circulating wild type strains requires continuous strain surveillance.
KW - Gastroenteritis
KW - Genotype
KW - Molecular epidemiology
KW - VP4
KW - VP7
UR - http://www.scopus.com/inward/record.url?scp=84865185482&partnerID=8YFLogxK
U2 - 10.1016/j.jcv.2012.06.016
DO - 10.1016/j.jcv.2012.06.016
M3 - Article
C2 - 22841750
AN - SCOPUS:84865185482
SN - 1386-6532
VL - 55
SP - 140
EP - 146
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
IS - 2
ER -