Surveillance of circulating bordetella pertussis strains in Europe during 1998 to 2015

Alex Mikael Barkoff; Jussi Mertsola; Denis Pierard; Tine Dalby; Silje Vermedal Hoegh; Sophie Guillot; Paola Stefanelli; Marjolein Van Gent; Guy Berbers; Didrik F. Vestrheim; Margrethe Greve-Isdahl; Lena Wehlin; Margaretha Ljungman; Norman K. Fry; Kevin Markey; Kari Auranen; Qiushui Hea

doi:10.1128/JCM.01998-17

Surveillance of circulating bordetella pertussis strains in Europe during 1998 to 2015

Alex Mikael Barkoff, Jussi Mertsola, Denis Pierard, Tine Dalby, Silje Vermedal Hoegh, Sophie Guillot, Paola Stefanelli, Marjolein Van Gent, Guy Berbers, Didrik F. Vestrheim, Margrethe Greve-Isdahl, Lena Wehlin, Margaretha Ljungman, Norman K. Fry, Kevin Markey, Kari Auranen, Qiushui Hea^*

^*Corresponding author for this work

Immunisation Hepatitis and Blood Safety

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

One reason for increased pertussis incidence is the adaptation of Bordetella pertussis to vaccine-induced immunity by modulating its genomic structure. This study, EUpert IV, includes 265 isolates collected from nine European countries during 2012 to 2015 (n 265) and compares the results to previous EUpert I to III studies (1998 to 2009). The analyses included genotyping, serotyping, pulsed-field gel electrophoresis (PFGE), and multilocus variable-number tandem-repeat analysis (MLVA). Genotyping results showed only small variations among the common virulence genes of B. pertussis. The frequencies of serotypes Fim2 and Fim3 varied among the four collections. Genomic analyses showed that MLVA type 27 increased to 80% between the periods of 1998 to 2001 and 2012 to 2015. Two PFGE profiles, BpSR3 (29.4%) and BpSR10 (27.2%), constituted more than 50% of the circulating isolates in the present collection. Our study indicates that the European B. pertussis population is changing and became more homogenous after the introduction of acellular pertussis vaccines.

Original language	English
Article number	e01998-17
Journal	Journal of Clinical Microbiology
Volume	56
Issue number	5
DOIs	https://doi.org/10.1128/JCM.01998-17
Publication status	Published - May 2018

Bibliographical note

Funding Information:
eDepartment of Clinical Microbiology, Odense, University Hospital, Odense, Denmark fNational Reference Center of Whooping Cough and Other Bordetelloses, Institut Pasteur, Paris, France gDepartment of Infectious Diseases, Istituto Superiore di Sanità, Rome, Italy hCentre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands

Publisher Copyright:
Copyright © 2018 American Society for Microbiology. All Rights Reserved.

Keywords

Bordetella pertussis
Europe
Genotyping
MLVA
PFGE
Serotyping

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/JCM.01998-17

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Barkoff, A. M., Mertsola, J., Pierard, D., Dalby, T., Hoegh, S. V., Guillot, S., Stefanelli, P., Van Gent, M., Berbers, G., Vestrheim, D. F., Greve-Isdahl, M., Wehlin, L., Ljungman, M., Fry, N. K., Markey, K., Auranen, K., & Hea, Q. (2018). Surveillance of circulating bordetella pertussis strains in Europe during 1998 to 2015. Journal of Clinical Microbiology, 56(5), [e01998-17]. https://doi.org/10.1128/JCM.01998-17

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title = "Surveillance of circulating bordetella pertussis strains in Europe during 1998 to 2015",

abstract = "One reason for increased pertussis incidence is the adaptation of Bordetella pertussis to vaccine-induced immunity by modulating its genomic structure. This study, EUpert IV, includes 265 isolates collected from nine European countries during 2012 to 2015 (n 265) and compares the results to previous EUpert I to III studies (1998 to 2009). The analyses included genotyping, serotyping, pulsed-field gel electrophoresis (PFGE), and multilocus variable-number tandem-repeat analysis (MLVA). Genotyping results showed only small variations among the common virulence genes of B. pertussis. The frequencies of serotypes Fim2 and Fim3 varied among the four collections. Genomic analyses showed that MLVA type 27 increased to 80% between the periods of 1998 to 2001 and 2012 to 2015. Two PFGE profiles, BpSR3 (29.4%) and BpSR10 (27.2%), constituted more than 50% of the circulating isolates in the present collection. Our study indicates that the European B. pertussis population is changing and became more homogenous after the introduction of acellular pertussis vaccines.",

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author = "Barkoff, {Alex Mikael} and Jussi Mertsola and Denis Pierard and Tine Dalby and Hoegh, {Silje Vermedal} and Sophie Guillot and Paola Stefanelli and {Van Gent}, Marjolein and Guy Berbers and Vestrheim, {Didrik F.} and Margrethe Greve-Isdahl and Lena Wehlin and Margaretha Ljungman and Fry, {Norman K.} and Kevin Markey and Kari Auranen and Qiushui Hea",

note = "Funding Information: eDepartment of Clinical Microbiology, Odense, University Hospital, Odense, Denmark fNational Reference Center of Whooping Cough and Other Bordetelloses, Institut Pasteur, Paris, France gDepartment of Infectious Diseases, Istituto Superiore di Sanit{\`a}, Rome, Italy hCentre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands Publisher Copyright: Copyright {\textcopyright} 2018 American Society for Microbiology. All Rights Reserved.",

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doi = "10.1128/JCM.01998-17",

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Barkoff, AM, Mertsola, J, Pierard, D, Dalby, T, Hoegh, SV, Guillot, S, Stefanelli, P, Van Gent, M, Berbers, G, Vestrheim, DF, Greve-Isdahl, M, Wehlin, L, Ljungman, M, Fry, NK, Markey, K, Auranen, K & Hea, Q 2018, 'Surveillance of circulating bordetella pertussis strains in Europe during 1998 to 2015', Journal of Clinical Microbiology, vol. 56, no. 5, e01998-17. https://doi.org/10.1128/JCM.01998-17

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AU - Barkoff, Alex Mikael

AU - Mertsola, Jussi

AU - Pierard, Denis

AU - Dalby, Tine

AU - Hoegh, Silje Vermedal

AU - Guillot, Sophie

AU - Stefanelli, Paola

AU - Van Gent, Marjolein

AU - Berbers, Guy

AU - Vestrheim, Didrik F.

AU - Greve-Isdahl, Margrethe

AU - Wehlin, Lena

AU - Ljungman, Margaretha

AU - Fry, Norman K.

AU - Markey, Kevin

AU - Auranen, Kari

AU - Hea, Qiushui

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PY - 2018/5

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N2 - One reason for increased pertussis incidence is the adaptation of Bordetella pertussis to vaccine-induced immunity by modulating its genomic structure. This study, EUpert IV, includes 265 isolates collected from nine European countries during 2012 to 2015 (n 265) and compares the results to previous EUpert I to III studies (1998 to 2009). The analyses included genotyping, serotyping, pulsed-field gel electrophoresis (PFGE), and multilocus variable-number tandem-repeat analysis (MLVA). Genotyping results showed only small variations among the common virulence genes of B. pertussis. The frequencies of serotypes Fim2 and Fim3 varied among the four collections. Genomic analyses showed that MLVA type 27 increased to 80% between the periods of 1998 to 2001 and 2012 to 2015. Two PFGE profiles, BpSR3 (29.4%) and BpSR10 (27.2%), constituted more than 50% of the circulating isolates in the present collection. Our study indicates that the European B. pertussis population is changing and became more homogenous after the introduction of acellular pertussis vaccines.

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KW - Genotyping

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KW - PFGE

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ER -

Surveillance of circulating bordetella pertussis strains in Europe during 1998 to 2015

Abstract

Bibliographical note

Keywords

UN SDGs

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