35S-sulphate labelling of glycoproteins and glycolipids in rabbit small intestinal brush borders

J. R. Cooper; P. W. Kent

doi:10.1016/0006-291X(78)91035-5

³⁵S-sulphate labelling of glycoproteins and glycolipids in rabbit small intestinal brush borders

J. R. Cooper^*, P. W. Kent

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

Isolated brush borders become labelled within 7.5 hours following injection of Na₂³⁵SO₄ into rabbits. The label is present in glycoprotein (73%) and in an hydrophobic glycolipid fraction (24%). T.l.c. and DEAE-cellulose chromatography indicate the presence of one principal sulphated glycolipid in which the sugars are glucose, galactose and glucosamine (1:2:2). Sialic acid residues are absent from the glycoproteins and are found only in minor ganglioside components. Ester sulphate is considered to contribute significantly to the anionic character of the membrane.

Original language	English
Pages (from-to)	622-627
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	83
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(78)91035-5
Publication status	Published - 28 Jul 1978

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title = "35S-sulphate labelling of glycoproteins and glycolipids in rabbit small intestinal brush borders",

abstract = "Isolated brush borders become labelled within 7.5 hours following injection of Na235SO4 into rabbits. The label is present in glycoprotein (73%) and in an hydrophobic glycolipid fraction (24%). T.l.c. and DEAE-cellulose chromatography indicate the presence of one principal sulphated glycolipid in which the sugars are glucose, galactose and glucosamine (1:2:2). Sialic acid residues are absent from the glycoproteins and are found only in minor ganglioside components. Ester sulphate is considered to contribute significantly to the anionic character of the membrane.",

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AU - Cooper, J. R.

AU - Kent, P. W.

PY - 1978/7/28

Y1 - 1978/7/28

N2 - Isolated brush borders become labelled within 7.5 hours following injection of Na235SO4 into rabbits. The label is present in glycoprotein (73%) and in an hydrophobic glycolipid fraction (24%). T.l.c. and DEAE-cellulose chromatography indicate the presence of one principal sulphated glycolipid in which the sugars are glucose, galactose and glucosamine (1:2:2). Sialic acid residues are absent from the glycoproteins and are found only in minor ganglioside components. Ester sulphate is considered to contribute significantly to the anionic character of the membrane.

AB - Isolated brush borders become labelled within 7.5 hours following injection of Na235SO4 into rabbits. The label is present in glycoprotein (73%) and in an hydrophobic glycolipid fraction (24%). T.l.c. and DEAE-cellulose chromatography indicate the presence of one principal sulphated glycolipid in which the sugars are glucose, galactose and glucosamine (1:2:2). Sialic acid residues are absent from the glycoproteins and are found only in minor ganglioside components. Ester sulphate is considered to contribute significantly to the anionic character of the membrane.

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ER -

³⁵S-sulphate labelling of glycoproteins and glycolipids in rabbit small intestinal brush borders

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