TY - JOUR
T1 - Subtypes of Cryptosporidium parvum in humans and disease risk
AU - Hunter, Paul R.
AU - Hadfield, Stephen J.
AU - Wilkinson, Dawn
AU - Lake, Iain R.
AU - Harrison, Florence C.D.
AU - Chalmers, Rachel M.
PY - 2007/1
Y1 - 2007/1
N2 - The 2 main species of Cryptosporidium that infect humans are Cryptosporidium hominis and C. parvum. Here, multilocus fragment analysis of 3 microsatellite loci (ML1, ML2, and gp60) was used to subtype strains from sporadic cases of cryptosporidiosis in Wales and northwest England. Of 72 strains of C. parvum, 63 were typeable at all 3 loci, forming 31 subtypes. These strains formed 3 broad clusters, representing 74.6%, 20.6%, and 4.8% of typeable strains. Of 118 C. hominis strains, 106 were typeable at all 3 loci, forming 9 subtypes; however, 90% belonged to the same subtype. Analysis with epidemiologic data found an association between strains from casepatients who reported contact with farm animals and individual C. parvum microsatellite alleles. The strongest association was with ML1; all strains from case-patients that reported farm animal contact had the same allele (ML1-242). Microsatellite typing of C. parvum provides valuable additional information on the epidemiology of this pathogen.
AB - The 2 main species of Cryptosporidium that infect humans are Cryptosporidium hominis and C. parvum. Here, multilocus fragment analysis of 3 microsatellite loci (ML1, ML2, and gp60) was used to subtype strains from sporadic cases of cryptosporidiosis in Wales and northwest England. Of 72 strains of C. parvum, 63 were typeable at all 3 loci, forming 31 subtypes. These strains formed 3 broad clusters, representing 74.6%, 20.6%, and 4.8% of typeable strains. Of 118 C. hominis strains, 106 were typeable at all 3 loci, forming 9 subtypes; however, 90% belonged to the same subtype. Analysis with epidemiologic data found an association between strains from casepatients who reported contact with farm animals and individual C. parvum microsatellite alleles. The strongest association was with ML1; all strains from case-patients that reported farm animal contact had the same allele (ML1-242). Microsatellite typing of C. parvum provides valuable additional information on the epidemiology of this pathogen.
UR - http://www.scopus.com/inward/record.url?scp=33846034005&partnerID=8YFLogxK
U2 - 10.3201/eid1301.060481
DO - 10.3201/eid1301.060481
M3 - Article
C2 - 17370519
AN - SCOPUS:33846034005
SN - 1080-6040
VL - 13
SP - 82
EP - 88
JO - Emerging Infectious Diseases
JF - Emerging Infectious Diseases
IS - 1
ER -
