TY - JOUR
T1 - Substitutions in position 222 of haemagglutinin of pandemic influenza A (H1N1) 2009 viruses in Spain
AU - Ledesma, Juan
AU - Pozo, Francisco
AU - Ruiz, Mercedes Pérez
AU - Navarro, Jose María
AU - Piñeiro, Luis
AU - Montes, Milagros
AU - Castro, Sonia Pérez
AU - Fernández, Jonathan Suárez
AU - Costa, Juan García
AU - Fernández, Mirian
AU - Galán, Juan Carlos
AU - Cuevas, María Teresa
AU - Casas, Inmaculada
AU - Breña, Pilar Pérez
PY - 2011/5
Y1 - 2011/5
N2 - Background: A change of aspartic acid (D) to glycine (G) at position 222 in the haemagglutinin (HA) protein of pandemic influenza A (H1N1) 2009 viruses was described in Norway on November 2009 with considerable frequency in fatal and severe cases. This change was detected in other countries and was related only with severe disease. Other substitutions to glutamic acid (E) or asparagine (N) at position 222 were detected among pandemic viruses but it is unclear what implications might have in terms of severity. Objectives: To analyse the appearance of amino acid substitutions at position 222 in the HA protein of circulating viruses in Spain and to determine their relationships with the disease symptoms observed. Study design: Pandemic influenza A (H1N1) 2009 viruses detected in respiratory samples of 273 severe and 533 non-severe cases from different Spanish regions were selected for sequencing of a partial segment of HA1 subunit and studied to monitor substitutions at position 222. Results: D222G substitution was only detected in viruses from 14 severe cases (5.12%). D222E was found in viruses from 47 severe (17.21%) and from 52 non-severe cases (9.75%). D222N occurred in viruses from 3 additional severe cases (0.37%). Conclusion: Appearance of D222G and D222E substitution in HA of pandemic influenza A (H1N1) viruses circulating in Spain might be related with severe respiratory disease.
AB - Background: A change of aspartic acid (D) to glycine (G) at position 222 in the haemagglutinin (HA) protein of pandemic influenza A (H1N1) 2009 viruses was described in Norway on November 2009 with considerable frequency in fatal and severe cases. This change was detected in other countries and was related only with severe disease. Other substitutions to glutamic acid (E) or asparagine (N) at position 222 were detected among pandemic viruses but it is unclear what implications might have in terms of severity. Objectives: To analyse the appearance of amino acid substitutions at position 222 in the HA protein of circulating viruses in Spain and to determine their relationships with the disease symptoms observed. Study design: Pandemic influenza A (H1N1) 2009 viruses detected in respiratory samples of 273 severe and 533 non-severe cases from different Spanish regions were selected for sequencing of a partial segment of HA1 subunit and studied to monitor substitutions at position 222. Results: D222G substitution was only detected in viruses from 14 severe cases (5.12%). D222E was found in viruses from 47 severe (17.21%) and from 52 non-severe cases (9.75%). D222N occurred in viruses from 3 additional severe cases (0.37%). Conclusion: Appearance of D222G and D222E substitution in HA of pandemic influenza A (H1N1) viruses circulating in Spain might be related with severe respiratory disease.
KW - D222G substitution
KW - Haemagglutinin sequencing
KW - Pandemic influenza A (H1N1) 2009 virus
KW - Severe respiratory infection
UR - http://www.scopus.com/inward/record.url?scp=79953745452&partnerID=8YFLogxK
U2 - 10.1016/j.jcv.2011.01.020
DO - 10.1016/j.jcv.2011.01.020
M3 - Article
C2 - 21353628
AN - SCOPUS:79953745452
SN - 1386-6532
VL - 51
SP - 75
EP - 78
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
IS - 1
ER -
