Structure and function of a Clostridium difficile sortase enzyme

Christopher J. Chambers, April K. Roberts, Clifford Shone*, K. Ravi Acharya

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Sortase enzymes are responsible for covalent anchoring of specific proteins to the peptidoglycan of the cell wall of gram-positive bacteria. In some gram-positive bacteria (e.g. Staphylococcus aureus), sortases have been found to be essential for pathogenesis and their inhibitors are under development as potential novel therapeutics. Here we provide the first report on the structural characterisation of the C. difficile sortase. An active site mutant was crystallised and its structure determined to 2.55 Å by X-ray diffraction to provide structural insight into its catalytic mechanism. In order to elucidate the role of the sortase in the cell wall biogenesis, a C. difficile sortase knockout strain was constructed by intron mutagenesis. Characterisation of this mutant led to the discovery that the putative adhesin CD0386 is anchored to the peptidoglycan of C. difficile by the sortase SrtB and that an SPKTG peptide motif is involved in the transpeptidation reaction with the C. difficile peptidoglycan. In an animal model for C. difficile infection, the SrtB mutant caused disease at a similar rate of onset as the wild type strain. In conclusion, our detailed study shows that the SrtB enzyme from C. difficile does not play an essential role in pathogenesis.

Original languageEnglish
Article number9449
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 24 Mar 2015

Bibliographical note

Funding Information:
We thank the scientists at PX station I04-1 of Diamond Light Source, Didcot, Oxfordshire (UK), for their support during X-ray diffraction data collection. We also thank Anneke Lubben (University of Bath) for her assistance in acquisition and interpretation of LC-MS data. Special thanks are owed to Jonathan Kirby and Helen Ahern for their assistance with the Clostron system, to Nethaji Thiyagarajan for his assistance with computing and William Bradshaw for help with the final round of refinement, validation and PDB deposition of the structure. C.J.C. was supported by a joint post-graduate studentship by the Public Health England and University of Bath (UK) and the Medical Research Council (UK) project grant (MK/K027123/1) to K.R.A. and C.C.S.

Fingerprint

Dive into the research topics of 'Structure and function of a Clostridium difficile sortase enzyme'. Together they form a unique fingerprint.

Cite this