Structure and conformation of a novel genetically engineered polysaccharide P2

Ian J. Colquhoun; Andrew J. Jay; John Eagles; Victor J. Morris; Kirstin J. Edwards; Annette M. Griffin; Mike J. Gasson

doi:10.1016/S0008-6215(00)00293-7

Structure and conformation of a novel genetically engineered polysaccharide P2

Ian J. Colquhoun, Andrew J. Jay^*, John Eagles, Victor J. Morris, Kirstin J. Edwards, Annette M. Griffin, Mike J. Gasson

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

A new exocellular polysaccharide (P2) has been produced by the manipulation of a glycosyl transferase gene (aceP) involved in the biosynthesis of the polysaccharide acetan by the bacterium Acetobacter xylinum strain CKE5. The P2 polysaccharide has been studied by methylation analysis, reductive cleavage, and ¹H and ¹³C NMR spectroscopy. The data are consistent with the structure predicted when the aceP gene is deactivated: The effect of cooling on proton NMR line width indicates a coil-helix transition in P2 at about 70°C.

Original language	English
Pages (from-to)	325-333
Number of pages	9
Journal	Carbohydrate Research
Volume	330
Issue number	3
DOIs	https://doi.org/10.1016/S0008-6215(00)00293-7
Publication status	Published - 15 Feb 2001
Externally published	Yes

Bibliographical note

Funding Information:
This work was funded by the Biotechnology and Biological Sciences Research Council, UK.

Keywords

Acetobacter xylinum
Methylation
NMR
Polysaccharide
aceP gene

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10.1016/S0008-6215(00)00293-7

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title = "Structure and conformation of a novel genetically engineered polysaccharide P2",

abstract = "A new exocellular polysaccharide (P2) has been produced by the manipulation of a glycosyl transferase gene (aceP) involved in the biosynthesis of the polysaccharide acetan by the bacterium Acetobacter xylinum strain CKE5. The P2 polysaccharide has been studied by methylation analysis, reductive cleavage, and 1H and 13C NMR spectroscopy. The data are consistent with the structure predicted when the aceP gene is deactivated: The effect of cooling on proton NMR line width indicates a coil-helix transition in P2 at about 70°C.",

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AU - Colquhoun, Ian J.

AU - Jay, Andrew J.

AU - Eagles, John

AU - Morris, Victor J.

AU - Edwards, Kirstin J.

AU - Griffin, Annette M.

AU - Gasson, Mike J.

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AB - A new exocellular polysaccharide (P2) has been produced by the manipulation of a glycosyl transferase gene (aceP) involved in the biosynthesis of the polysaccharide acetan by the bacterium Acetobacter xylinum strain CKE5. The P2 polysaccharide has been studied by methylation analysis, reductive cleavage, and 1H and 13C NMR spectroscopy. The data are consistent with the structure predicted when the aceP gene is deactivated: The effect of cooling on proton NMR line width indicates a coil-helix transition in P2 at about 70°C.

KW - Acetobacter xylinum

KW - Methylation

KW - NMR

KW - Polysaccharide

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Structure and conformation of a novel genetically engineered polysaccharide P2

Abstract

Bibliographical note

Keywords

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