Strain variation in Campylobacter pylori detected by numerical analysis of one-dimensional electrophoretic protein patterns

Robert Owen*, M. Costas, D. D. Morgan, S. L.W. On, L. R. Hill, A. D. Pearson, D. R. Morgan

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    21 Citations (Scopus)

    Abstract

    A total of 21 clinical isolates of Campylobacter pylori from Peru and the United Kingdom and two reference strains (from Australia), including the type strain (NCTC 11637T), were characterized by high resolution one-dimensional SDS-polyacrylamide gel electrophoresis of cellular proteins. The protein patterns contained more than 40 discrete bands and the approximate molecular weights of the major bands were 22, 27, 46, 57, 60, 65 and 93 kD. The total patterns were used as the basis of numerical analysis. Most strains were clustered in four phenons at 91% similarity with the exception of six ungrouped strains. Overall similarity was high with all strains linked in the phenogram at ≥81%. Variation among strains was attributable principally to qualitative and quantitative band differences in the 47 to 56 kD (hypervariable) region of the C. pylori protein profile. From the analysis, ten different electropherotypes (EP-types) were identified. We demonstrated that differences were detectable among isolates from widely separated geographical locations as well as from the same location, although multiple isolates from two Peruvian patients had the same electropherotype. Our results indicate that determination of protein profiles provides the basis of a reproducible method for characterization of C. pylori isolates.

    Original languageEnglish
    Pages (from-to)253-267
    Number of pages15
    JournalAntonie van Leeuwenhoek
    Volume55
    Issue number3
    DOIs
    Publication statusPublished - Mar 1989

    Keywords

    • Campylobacter pylori
    • computer analysis
    • electrophoresis
    • Protein profiles

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