Simple and universal HPLC-UV method to determine cimetidine, ranitidine, famotidine and nizatidine in urine: Application to the analysis of ranitidine and its metabolites in human volunteers

Diane A.I. Ashiru; Rajesh Patel; Abdul W. Basit

doi:10.1016/j.jchromb.2007.10.029

Simple and universal HPLC-UV method to determine cimetidine, ranitidine, famotidine and nizatidine in urine: Application to the analysis of ranitidine and its metabolites in human volunteers

Diane A.I. Ashiru, Rajesh Patel, Abdul W. Basit^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

69 Citations (Scopus)

Abstract

A validated, simple and universal HPLC-UV method for the determination of cimetidine, famotidine, nizatidine and ranitidine in human urine is presented. This is the first single HPLC method reported for the analysis of all four H₂ antagonists in human biological samples. This method was also utilized for the analysis of ranitidine and its metabolites in human urine. All calibration curves showed good linear regression (r² > 0.9960) within test ranges. The method showed good precision and accuracy with overall intra- and inter-day variations of 0.2-13.6% and 0.2-12.1%, respectively. Separation of ranitidine and its metabolites using this assay provided significantly improved resolution, precision and accuracy compared to previously reported methods. The assay was successfully applied to a human volunteer study using ranitidine as the model compound.

Original language	English
Pages (from-to)	235-240
Number of pages	6
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	860
Issue number	2
DOIs	https://doi.org/10.1016/j.jchromb.2007.10.029
Publication status	Published - 15 Dec 2007
Externally published	Yes

Keywords

Bioavailability
Cimetidine
Famotidine
H antagonists
High performance liquid chromatography
Metabolism
Nizatidine
Ranitidine

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10.1016/j.jchromb.2007.10.029

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Ashiru, D. A. I., Patel, R., & Basit, A. W. (2007). Simple and universal HPLC-UV method to determine cimetidine, ranitidine, famotidine and nizatidine in urine: Application to the analysis of ranitidine and its metabolites in human volunteers. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 860(2), 235-240. https://doi.org/10.1016/j.jchromb.2007.10.029

Ashiru, Diane A.I. ; Patel, Rajesh ; Basit, Abdul W. / Simple and universal HPLC-UV method to determine cimetidine, ranitidine, famotidine and nizatidine in urine : Application to the analysis of ranitidine and its metabolites in human volunteers. In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2007 ; Vol. 860, No. 2. pp. 235-240.

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title = "Simple and universal HPLC-UV method to determine cimetidine, ranitidine, famotidine and nizatidine in urine: Application to the analysis of ranitidine and its metabolites in human volunteers",

abstract = "A validated, simple and universal HPLC-UV method for the determination of cimetidine, famotidine, nizatidine and ranitidine in human urine is presented. This is the first single HPLC method reported for the analysis of all four H2 antagonists in human biological samples. This method was also utilized for the analysis of ranitidine and its metabolites in human urine. All calibration curves showed good linear regression (r2 > 0.9960) within test ranges. The method showed good precision and accuracy with overall intra- and inter-day variations of 0.2-13.6% and 0.2-12.1%, respectively. Separation of ranitidine and its metabolites using this assay provided significantly improved resolution, precision and accuracy compared to previously reported methods. The assay was successfully applied to a human volunteer study using ranitidine as the model compound.",

keywords = "Bioavailability, Cimetidine, Famotidine, H antagonists, High performance liquid chromatography, Metabolism, Nizatidine, Ranitidine",

author = "Ashiru, {Diane A.I.} and Rajesh Patel and Basit, {Abdul W.}",

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Ashiru, DAI, Patel, R & Basit, AW 2007, 'Simple and universal HPLC-UV method to determine cimetidine, ranitidine, famotidine and nizatidine in urine: Application to the analysis of ranitidine and its metabolites in human volunteers', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 860, no. 2, pp. 235-240. https://doi.org/10.1016/j.jchromb.2007.10.029

Simple and universal HPLC-UV method to determine cimetidine, ranitidine, famotidine and nizatidine in urine: Application to the analysis of ranitidine and its metabolites in human volunteers. / Ashiru, Diane A.I.; Patel, Rajesh; Basit, Abdul W.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 860, No. 2, 15.12.2007, p. 235-240.

Research output: Contribution to journal › Article › peer-review

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T1 - Simple and universal HPLC-UV method to determine cimetidine, ranitidine, famotidine and nizatidine in urine

T2 - Application to the analysis of ranitidine and its metabolites in human volunteers

AU - Ashiru, Diane A.I.

AU - Patel, Rajesh

AU - Basit, Abdul W.

PY - 2007/12/15

Y1 - 2007/12/15

N2 - A validated, simple and universal HPLC-UV method for the determination of cimetidine, famotidine, nizatidine and ranitidine in human urine is presented. This is the first single HPLC method reported for the analysis of all four H2 antagonists in human biological samples. This method was also utilized for the analysis of ranitidine and its metabolites in human urine. All calibration curves showed good linear regression (r2 > 0.9960) within test ranges. The method showed good precision and accuracy with overall intra- and inter-day variations of 0.2-13.6% and 0.2-12.1%, respectively. Separation of ranitidine and its metabolites using this assay provided significantly improved resolution, precision and accuracy compared to previously reported methods. The assay was successfully applied to a human volunteer study using ranitidine as the model compound.

AB - A validated, simple and universal HPLC-UV method for the determination of cimetidine, famotidine, nizatidine and ranitidine in human urine is presented. This is the first single HPLC method reported for the analysis of all four H2 antagonists in human biological samples. This method was also utilized for the analysis of ranitidine and its metabolites in human urine. All calibration curves showed good linear regression (r2 > 0.9960) within test ranges. The method showed good precision and accuracy with overall intra- and inter-day variations of 0.2-13.6% and 0.2-12.1%, respectively. Separation of ranitidine and its metabolites using this assay provided significantly improved resolution, precision and accuracy compared to previously reported methods. The assay was successfully applied to a human volunteer study using ranitidine as the model compound.

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KW - Cimetidine

KW - Famotidine

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KW - High performance liquid chromatography

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Ashiru DAI, Patel R, Basit AW. Simple and universal HPLC-UV method to determine cimetidine, ranitidine, famotidine and nizatidine in urine: Application to the analysis of ranitidine and its metabolites in human volunteers. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2007 Dec 15;860(2):235-240. doi: 10.1016/j.jchromb.2007.10.029

Simple and universal HPLC-UV method to determine cimetidine, ranitidine, famotidine and nizatidine in urine: Application to the analysis of ranitidine and its metabolites in human volunteers

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