TY - JOUR
T1 - Serotype and genomic diversity of dengue virus during the 2023 outbreak in Pakistan reveals the circulation of genotype III of DENV-1 and cosmopolitan genotype of DENV-2
AU - Jamal, Zunera
AU - Haider, Syed Adnan
AU - Hakim, Rabia
AU - Humayun, Fahad
AU - Farooq, Muhammad Umar
AU - Ammar, Muhammad
AU - Afrough, Babak
AU - Inamdar, Leena
AU - Salman, Muhammad
AU - Umair, Massab
N1 - Publisher Copyright:
© 2024 Wiley Periodicals LLC.
PY - 2024/6/12
Y1 - 2024/6/12
N2 - Dengue, a mosquito-borne viral disease, poses a significant public health challenge in Pakistan, with a significant outbreak in 2023, prompting our investigation into the serotype and genomic diversity of the dengue virus (DENV). NS-1 positive blood samples from 153 patients were referred to the National Institute of Health, Pakistan, between July and October 2023. Among these, 98 (64.1%) tested positive using multiplex real-time PCR, with higher prevalence among males (65.8%) and individuals aged 31-40. Serotyping revealed DENV-1 as the predominant serotype (84.7%), followed by DENV-2 (15.3%). Whole-genome sequencing of 18 samples (DENV-1 = 17, DENV-2 = 01) showed that DENV-1 (genotype III) samples were closely related (>99%) to Pakistan outbreak samples (2022), and approx. > 98% with USA (2022), Singapore and China (2016), Bangladesh (2017), and Pakistan (2019). The DENV-2 sequence (cosmopolitan genotype; clade IVA) shared genetic similarity with Pakistan outbreak sequences (2022), approx. > 99% with China and Singapore (2018−2019) and showed divergence from Pakistan sequences (2008−2013). No coinfection with dengue serotypes or other viruses were observed. Comparisons with previous DENV-1 sequences highlighted genetic variations affecting viral replication efficiency (NS2B:K55R) and infectivity (E:M272T). These findings contribute to dengue epidemiology understanding and underscore the importance of ongoing genomic surveillance for future outbreak responses in Pakistan.
AB - Dengue, a mosquito-borne viral disease, poses a significant public health challenge in Pakistan, with a significant outbreak in 2023, prompting our investigation into the serotype and genomic diversity of the dengue virus (DENV). NS-1 positive blood samples from 153 patients were referred to the National Institute of Health, Pakistan, between July and October 2023. Among these, 98 (64.1%) tested positive using multiplex real-time PCR, with higher prevalence among males (65.8%) and individuals aged 31-40. Serotyping revealed DENV-1 as the predominant serotype (84.7%), followed by DENV-2 (15.3%). Whole-genome sequencing of 18 samples (DENV-1 = 17, DENV-2 = 01) showed that DENV-1 (genotype III) samples were closely related (>99%) to Pakistan outbreak samples (2022), and approx. > 98% with USA (2022), Singapore and China (2016), Bangladesh (2017), and Pakistan (2019). The DENV-2 sequence (cosmopolitan genotype; clade IVA) shared genetic similarity with Pakistan outbreak sequences (2022), approx. > 99% with China and Singapore (2018−2019) and showed divergence from Pakistan sequences (2008−2013). No coinfection with dengue serotypes or other viruses were observed. Comparisons with previous DENV-1 sequences highlighted genetic variations affecting viral replication efficiency (NS2B:K55R) and infectivity (E:M272T). These findings contribute to dengue epidemiology understanding and underscore the importance of ongoing genomic surveillance for future outbreak responses in Pakistan.
KW - DENV-1
KW - DENV-2
KW - Pakistan
KW - cosmopolitan genotype
KW - dengue outbreak 2023
KW - serotype
KW - whole genome sequencing
UR - http://www.scopus.com/inward/record.url?scp=85195854201&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/e749fda2-af38-38e7-8fe4-3d358090fb46/
U2 - 10.1002/jmv.29727
DO - 10.1002/jmv.29727
M3 - Article
C2 - 38864343
AN - SCOPUS:85195854201
SN - 0146-6615
VL - 96
JO - Journal of Medical Virology
JF - Journal of Medical Virology
IS - 6
M1 - e29727
ER -
