Selection and characterization of mutational resistance to aztreonam/avibactam in b-lactamase-producing Enterobacterales

Shazad Mushtaq, Anna Vickers, Nicholas Ellaby, Neil Woodford, David M. Livermore*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Background: Aztreonam/avibactam is being developed for its broad activity against carbapenemase-producing Enterobacterales, including those with metallo-b-lactamases (MBLs). Its potential to select resistance in target pathogens was explored. Findings are compared with previous data for ceftazidime/avibactam and ceftaroline/ avibactam. Methods: Single-step mutants were sought from 52 Enterobacterales with AmpC, ESBL, KPC, MBL and OXA-48like enzymes. Mutation frequencies were calculated. MICs were determined by CLSI agar dilution. Genomes were sequenced using Illumina methodology. Results: Irrespective of b-lactamase type and of whether avibactam was used at 1 or 4 mg/L, mutants could rarely be obtained at >4% the starting MIC, and most MIC rises were correspondingly small. Putative resistance (MIC >8 ! 4 mg/L) associated with changes to b-lactamases was seen only for mutants of AmpC, where it was associated with Asn346Tyr and Tyr150Cys substitutions. Asn346Tyr led to broad resistance to avibactam combinations; Tyr150Cys significantly affected only aztreonam/avibactam. MIC rises up to 4 ! 4 mg/L were seen for producers of mutant KPC-2 or -3 enzymes, and were associated with Trp105Arg, Ser106Pro and Ser109Pro substitutions, which all reduced the MICs of other b-lactams. For producers of other b-lactamase types, we largely found mutants with lesions in baeRS or envZ, putatively affecting drug accumulation. Single mutants had lesions in ampD, affecting AmpC expression or ftsI, encoding PBP3. Conclusions: The risk of mutational resistance to aztreonam/avibactam appears smaller than for ceftazidime/ avibactam, where Asp179Tyr arises readily in KPC enzymes, conferring frank resistance. Asn346 substitutions in AmpC enzymes may remain a risk, having been repeatedly selected with multiple avibactam combinations in vitro.

Original languageEnglish
Pages (from-to)98-111
Number of pages14
JournalJournal of Antimicrobial Chemotherapy
Volume77
Issue number1
DOIs
Publication statusPublished - 1 Jan 2022

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