Satellite cell myogenesis is highly stimulated by the kinase inhibitor iso-H7: Comparison with HA1004 and staurosporine effects

Catherine Lagord; Gilles Carpentier; Jerzy Moraczewski; Gabriel Pons; Fernando Climent; Isabelle Martelly

doi:10.1006/bbrc.1993.1306

Satellite cell myogenesis is highly stimulated by the kinase inhibitor iso-H7: Comparison with HA1004 and staurosporine effects

Catherine Lagord, Gilles Carpentier, Jerzy Moraczewski, Gabriel Pons, Fernando Climent, Isabelle Martelly^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Differentiation of rat satellite cells, measured by cell fusion into myotubes and isozymic conversion of creatine kinase and phosphoglycerate mutase, was shown to be highly increased in the presence of 1-(5- isoquinolinylsulfonyl)-3-methylpiperazine (iso-H7). This substance inhibited both protein kinase C (PKC) and cAMP-dependent protein kinase (PKA) activities with similar IC50 between 22 and 34 μM. Iso-H7, as well as the PKA inhibitor HA1004 increased myogenic differentiation without altering the proliferation of satellite cells, whereas the proliferation and the differentiation of these cells were inhibited by the PKC inhibitor staurosporine. Our results suggest a predominant negative control of PKA on satellite cell myogenesis.

Original language	English
Pages (from-to)	928-936
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	191
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.1993.1306
Publication status	Published - 31 Mar 1993
Externally published	Yes

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title = "Satellite cell myogenesis is highly stimulated by the kinase inhibitor iso-H7: Comparison with HA1004 and staurosporine effects",

abstract = "Differentiation of rat satellite cells, measured by cell fusion into myotubes and isozymic conversion of creatine kinase and phosphoglycerate mutase, was shown to be highly increased in the presence of 1-(5- isoquinolinylsulfonyl)-3-methylpiperazine (iso-H7). This substance inhibited both protein kinase C (PKC) and cAMP-dependent protein kinase (PKA) activities with similar IC50 between 22 and 34 μM. Iso-H7, as well as the PKA inhibitor HA1004 increased myogenic differentiation without altering the proliferation of satellite cells, whereas the proliferation and the differentiation of these cells were inhibited by the PKC inhibitor staurosporine. Our results suggest a predominant negative control of PKA on satellite cell myogenesis.",

author = "Catherine Lagord and Gilles Carpentier and Jerzy Moraczewski and Gabriel Pons and Fernando Climent and Isabelle Martelly",

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T1 - Satellite cell myogenesis is highly stimulated by the kinase inhibitor iso-H7

T2 - Comparison with HA1004 and staurosporine effects

AU - Lagord, Catherine

AU - Carpentier, Gilles

AU - Moraczewski, Jerzy

AU - Pons, Gabriel

AU - Climent, Fernando

AU - Martelly, Isabelle

PY - 1993/3/31

Y1 - 1993/3/31

N2 - Differentiation of rat satellite cells, measured by cell fusion into myotubes and isozymic conversion of creatine kinase and phosphoglycerate mutase, was shown to be highly increased in the presence of 1-(5- isoquinolinylsulfonyl)-3-methylpiperazine (iso-H7). This substance inhibited both protein kinase C (PKC) and cAMP-dependent protein kinase (PKA) activities with similar IC50 between 22 and 34 μM. Iso-H7, as well as the PKA inhibitor HA1004 increased myogenic differentiation without altering the proliferation of satellite cells, whereas the proliferation and the differentiation of these cells were inhibited by the PKC inhibitor staurosporine. Our results suggest a predominant negative control of PKA on satellite cell myogenesis.

AB - Differentiation of rat satellite cells, measured by cell fusion into myotubes and isozymic conversion of creatine kinase and phosphoglycerate mutase, was shown to be highly increased in the presence of 1-(5- isoquinolinylsulfonyl)-3-methylpiperazine (iso-H7). This substance inhibited both protein kinase C (PKC) and cAMP-dependent protein kinase (PKA) activities with similar IC50 between 22 and 34 μM. Iso-H7, as well as the PKA inhibitor HA1004 increased myogenic differentiation without altering the proliferation of satellite cells, whereas the proliferation and the differentiation of these cells were inhibited by the PKC inhibitor staurosporine. Our results suggest a predominant negative control of PKA on satellite cell myogenesis.

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Satellite cell myogenesis is highly stimulated by the kinase inhibitor iso-H7: Comparison with HA1004 and staurosporine effects

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