This study was designed to investigate whether saliva could be a feasible alternative to serum for the diagnosis of recent measles infection in a clinic setting. Forty-two paired blood and saliva samples collected 1 to 16 days after onset of illness from 29 patients with clinical measles were tested for specific immunoglobulin (Ig) M by antibody-capture radioimmunoassay. Measles IgM was detected in all serum samples and in 39 (92.9%) saliva specimens. Between 1 and 3 weeks after illness onset, virus-specific IgM was detected in 100% of saliva samples. Measles IgM was also detected in 17 saliva specimens, not paired with blood samples, collected from study patients 5 days to 3 weeks after onset. Our results indicate that salivary IgM detection is a suitable non-invasive method for investigation of notified cases under conditions of routine clinic use.
|Number of pages||3|
|Journal||Transactions of the Royal Society of Tropical Medicine and Hygiene|
|Publication status||Published - 1998|
Bibliographical noteFunding Information:
Acknowledgements We thank the general practitioners from the Department of Infectious Diseases/University Federal Fluminense and Centro de Saude Santa Rosa, Niteroi, Rio de Janeiro, Brazil, for their help in collecting the saliva samples. We also thank MS Pamela Litton and Mr Rashpal Hunjan from Virus Reference DivisioniPHLS, London, UK, for conducting the measles assays, and the British Council and Conselho National de Pesquisa e Desenvolvimento (CNPq-grant No. 52-0689/96-8) for providing financial support for the research.
- Antibody capture radioimmunoassay