The use of molecular methods for rotavirus characterisation provides not only increased sensitivity for typing, but also allows accurate and more complete characterisation of strains, and the identification of putative reassortant strains. However, due to the constant accumulation of point mutations through genetic drift, and to the emergence of novel genotypes, possibly zoonotic transmission and subsequent reassortment, the reagents and methods used require close monitoring and updating. Methods and oligonucleotide primers are described to overcome failures to type G9, G10 and P rotavirus strains, and cross-reactivity identified between G10 and G3 rotaviruses.
Bibliographical noteFunding Information:
Support for this work was obtained through an MRC Research Training Fellowship awarded to Miren Iturriza-Gómara and a Wellcome Trust Trilateral Infectious Disease Inititative Grant (No. 063144).
- Oligonucleotide primers
- Rotavirus genotyping
- Rotavirus strains