Role of stress responses in human cell survival following exposure to ultraviolet C radiation

N. A. Cridland, M. C. Martin, K. Stevens, C. A. Baller, A. J. Pearson, C. M.H. Driscoll, R. D. Saunders*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Purpose: To investigate in human skin and other cells the role of tyrosine kinase and protein kinase-C (PKC) in eliciting cell-signalling responses to UV radiation (UVR) that affect the survival of irradiated cells. Materials and methods: The survival of HeLa S3 cells, NCTC 2544 human keratinocytes and A431 human epidermal carcinoma cells was measured following incubation with various tyrosine kinase or PKC inhibitors and exposure to UVC (254nm) radiation. In addition, Western blotting measured PKC isozyme expression in human keratinocytes following UVC exposure. Results: It was confirmed that inhibition of tyrosine kinase activation reduces the survival of UV-irradiated HeLa S3 cells. However, no effect was seen on the survival of either NCTC 2544 human keratinocytes or A431 human epidermal carcinoma cells. In contrast, specific inhibition of PKC reduced the survival of UV-irradiated keratinocytes but had no effect on HeLa cells. Comparison of the effects of different inhibitors in keratinocytes suggested that this effect was mediated mostly through PKCμ and PKCλ/ι. In addition, keratinocyte exposure to UVC induced large and temporally distinct increases in PKCμ and PKCλ/ι. Conclusions: The survival of NCTC 2544 keratinocytes, but not HeLa S3 cells, following UVC exposure is mediated by signalling through PKC, mostly PKCμ and PKCλ/ι. Further study is required to confirm these results in normal human keratinocytes.

Original languageEnglish
Pages (from-to)365-374
Number of pages10
JournalInternational Journal of Radiation Biology
Volume77
Issue number3
DOIs
Publication statusPublished - 2001

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