Role of IgA in the defense against respiratory infections: IgA deficient mice exhibited increased susceptibility to intranasal infection with Mycobacterium bovis BCG

Ariane Rodríguez; Anna Tjärnlund; Juraj Ivanji; Mahavir Singh; Irene García; Ann Williams; Philip D. Marsh; Marita Troye-Blomberg; Carmen Fernández

doi:10.1016/j.vaccine.2004.11.032

Role of IgA in the defense against respiratory infections: IgA deficient mice exhibited increased susceptibility to intranasal infection with Mycobacterium bovis BCG

Ariane Rodríguez^*, Anna Tjärnlund, Juraj Ivanji, Mahavir Singh, Irene García, Ann Williams, Philip D. Marsh, Marita Troye-Blomberg, Carmen Fernández

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

112 Citations (Scopus)

Abstract

IgA is the predominant Ig isotype in mucosal tissue and is believed to be involved in defense against viral and bacterial infections at these sites. Here, we examined the role of IgA in the protection against intranasal (i.n.) infection with Mycobacterium bovis Bacillus Calmette-Guérin (BCG). IgA deficient (IgA^-/-) mice and wild type non-targeted littermate (IgA^+/+) mice were immunized by i.n. route with the mycobacterium surface antigen PstS-1 formulated with cholera toxin (CT). Our data showed that IgA^-/- mice were more susceptible to BCG infection compared to IgA^+/+ mice, as revealed by the higher bacterial loads in the lungs and bronchoalveolar lavage (BAL). Analysis of the Ig levels and the antibody responses to PstS-1 showed that IgA^-/- mice had no detectable IgA either in the saliva or in the BAL. However, these mice displayed higher levels of total and specific IgM than IgA^+/+ mice in both mucosal fluids. More importantly, analysis of the cytokine responses revealed a reduction in the IFN-γ and TNF-α production in the lungs of IgA^-/- compared to IgA^+/+ mice. Altogether, our results suggest that IgA may play a role in protection against mycobacterial infections in the respiratory tract by blocking the pathogen entrance and/or by modulating the pro-inflammatory responses.

Original language	English
Pages (from-to)	2565-2572
Number of pages	8
Journal	Vaccine
Volume	23
Issue number	20
DOIs	https://doi.org/10.1016/j.vaccine.2004.11.032
Publication status	Published - 8 Apr 2005

Bibliographical note

Funding Information:
A. Rodriguez and A. Tjärnlund shared first authorship. M. Troye-Blomberg and C. Fernández shared senior authorship. We thank E. Julian, F. Dieli, A. Salerno, J. Tree, R. Reljic and M. Comini for helpful suggestions and discussions. We also are grateful to I. Mbawuike for providing the IgA deficient mice. We thank P.J. Cardona for conducting the challenge experiments with the virulent mycobacteria. This work was financially supported by the European Commission (Fifth Framework Program, Contract QLK2-1999-00367) and by the Hjärt-LungFonden.

Keywords

BCG
IgA
Mucosal immunity

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vaccine.2004.11.032

Cite this

Rodríguez, A., Tjärnlund, A., Ivanji, J., Singh, M., García, I., Williams, A., Marsh, P. D., Troye-Blomberg, M., & Fernández, C. (2005). Role of IgA in the defense against respiratory infections: IgA deficient mice exhibited increased susceptibility to intranasal infection with Mycobacterium bovis BCG. Vaccine, 23(20), 2565-2572. https://doi.org/10.1016/j.vaccine.2004.11.032

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title = "Role of IgA in the defense against respiratory infections: IgA deficient mice exhibited increased susceptibility to intranasal infection with Mycobacterium bovis BCG",

abstract = "IgA is the predominant Ig isotype in mucosal tissue and is believed to be involved in defense against viral and bacterial infections at these sites. Here, we examined the role of IgA in the protection against intranasal (i.n.) infection with Mycobacterium bovis Bacillus Calmette-Gu{\'e}rin (BCG). IgA deficient (IgA-/-) mice and wild type non-targeted littermate (IgA+/+) mice were immunized by i.n. route with the mycobacterium surface antigen PstS-1 formulated with cholera toxin (CT). Our data showed that IgA-/- mice were more susceptible to BCG infection compared to IgA+/+ mice, as revealed by the higher bacterial loads in the lungs and bronchoalveolar lavage (BAL). Analysis of the Ig levels and the antibody responses to PstS-1 showed that IgA-/- mice had no detectable IgA either in the saliva or in the BAL. However, these mice displayed higher levels of total and specific IgM than IgA+/+ mice in both mucosal fluids. More importantly, analysis of the cytokine responses revealed a reduction in the IFN-γ and TNF-α production in the lungs of IgA-/- compared to IgA+/+ mice. Altogether, our results suggest that IgA may play a role in protection against mycobacterial infections in the respiratory tract by blocking the pathogen entrance and/or by modulating the pro-inflammatory responses.",

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author = "Ariane Rodr{\'i}guez and Anna Tj{\"a}rnlund and Juraj Ivanji and Mahavir Singh and Irene Garc{\'i}a and Ann Williams and Marsh, {Philip D.} and Marita Troye-Blomberg and Carmen Fern{\'a}ndez",

note = "Funding Information: A. Rodriguez and A. Tj{\"a}rnlund shared first authorship. M. Troye-Blomberg and C. Fern{\'a}ndez shared senior authorship. We thank E. Julian, F. Dieli, A. Salerno, J. Tree, R. Reljic and M. Comini for helpful suggestions and discussions. We also are grateful to I. Mbawuike for providing the IgA deficient mice. We thank P.J. Cardona for conducting the challenge experiments with the virulent mycobacteria. This work was financially supported by the European Commission (Fifth Framework Program, Contract QLK2-1999-00367) and by the Hj{\"a}rt-LungFonden.",

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Rodríguez, A, Tjärnlund, A, Ivanji, J, Singh, M, García, I, Williams, A, Marsh, PD, Troye-Blomberg, M & Fernández, C 2005, 'Role of IgA in the defense against respiratory infections: IgA deficient mice exhibited increased susceptibility to intranasal infection with Mycobacterium bovis BCG', Vaccine, vol. 23, no. 20, pp. 2565-2572. https://doi.org/10.1016/j.vaccine.2004.11.032

TY - JOUR

T1 - Role of IgA in the defense against respiratory infections

T2 - IgA deficient mice exhibited increased susceptibility to intranasal infection with Mycobacterium bovis BCG

AU - Rodríguez, Ariane

AU - Tjärnlund, Anna

AU - Ivanji, Juraj

AU - Singh, Mahavir

AU - García, Irene

AU - Williams, Ann

AU - Marsh, Philip D.

AU - Troye-Blomberg, Marita

AU - Fernández, Carmen

N1 - Funding Information: A. Rodriguez and A. Tjärnlund shared first authorship. M. Troye-Blomberg and C. Fernández shared senior authorship. We thank E. Julian, F. Dieli, A. Salerno, J. Tree, R. Reljic and M. Comini for helpful suggestions and discussions. We also are grateful to I. Mbawuike for providing the IgA deficient mice. We thank P.J. Cardona for conducting the challenge experiments with the virulent mycobacteria. This work was financially supported by the European Commission (Fifth Framework Program, Contract QLK2-1999-00367) and by the Hjärt-LungFonden.

PY - 2005/4/8

Y1 - 2005/4/8

N2 - IgA is the predominant Ig isotype in mucosal tissue and is believed to be involved in defense against viral and bacterial infections at these sites. Here, we examined the role of IgA in the protection against intranasal (i.n.) infection with Mycobacterium bovis Bacillus Calmette-Guérin (BCG). IgA deficient (IgA-/-) mice and wild type non-targeted littermate (IgA+/+) mice were immunized by i.n. route with the mycobacterium surface antigen PstS-1 formulated with cholera toxin (CT). Our data showed that IgA-/- mice were more susceptible to BCG infection compared to IgA+/+ mice, as revealed by the higher bacterial loads in the lungs and bronchoalveolar lavage (BAL). Analysis of the Ig levels and the antibody responses to PstS-1 showed that IgA-/- mice had no detectable IgA either in the saliva or in the BAL. However, these mice displayed higher levels of total and specific IgM than IgA+/+ mice in both mucosal fluids. More importantly, analysis of the cytokine responses revealed a reduction in the IFN-γ and TNF-α production in the lungs of IgA-/- compared to IgA+/+ mice. Altogether, our results suggest that IgA may play a role in protection against mycobacterial infections in the respiratory tract by blocking the pathogen entrance and/or by modulating the pro-inflammatory responses.

AB - IgA is the predominant Ig isotype in mucosal tissue and is believed to be involved in defense against viral and bacterial infections at these sites. Here, we examined the role of IgA in the protection against intranasal (i.n.) infection with Mycobacterium bovis Bacillus Calmette-Guérin (BCG). IgA deficient (IgA-/-) mice and wild type non-targeted littermate (IgA+/+) mice were immunized by i.n. route with the mycobacterium surface antigen PstS-1 formulated with cholera toxin (CT). Our data showed that IgA-/- mice were more susceptible to BCG infection compared to IgA+/+ mice, as revealed by the higher bacterial loads in the lungs and bronchoalveolar lavage (BAL). Analysis of the Ig levels and the antibody responses to PstS-1 showed that IgA-/- mice had no detectable IgA either in the saliva or in the BAL. However, these mice displayed higher levels of total and specific IgM than IgA+/+ mice in both mucosal fluids. More importantly, analysis of the cytokine responses revealed a reduction in the IFN-γ and TNF-α production in the lungs of IgA-/- compared to IgA+/+ mice. Altogether, our results suggest that IgA may play a role in protection against mycobacterial infections in the respiratory tract by blocking the pathogen entrance and/or by modulating the pro-inflammatory responses.

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KW - Mucosal immunity

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AN - SCOPUS:15044347334

SN - 0264-410X

VL - 23

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ER -

Role of IgA in the defense against respiratory infections: IgA deficient mice exhibited increased susceptibility to intranasal infection with Mycobacterium bovis BCG

Abstract

Bibliographical note

Keywords

UN SDGs

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