Risk factors for Group B Streptococcus colonisation and disease in Gambian women and their infants

K. Le Doare*, S. Jarju, S. Darboe, F. Warburton, Andrew Gorringe, P. T. Heath, B. Kampmann

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

49 Citations (Scopus)

Abstract

Objectives: To determine risk factors for GBS colonisation in Gambian mothers and in their infants from birth to day 60-89 of age. Methods: Swabs and breastmilk from mothers/infant pairs were collected and cultured on selective agar. Negative samples were analysed for GBS DNA via real-time PCR. Positive isolates were serotyped using multiplex PCR and gel-agarose electrophoresis. Results: Seven hundred and fifty women/infant pairs were recruited. 253 women (33.7%) were GBS-colonised at delivery. The predominant serotypes were: V (55%), II (16%), III (10%), Ia (8%) and Ib (8%). 186 infants were colonised (24.8%) at birth, 181 (24.1%) at 6 days and 96 at day 60-89 (14%). Infants born before 34 weeks of gestation and to women with rectovaginal and breastmilk colonisation at delivery had increased odds of GBS colonisation at birth. Season of birth was associated with increased odds of persistent infant GBS colonisation (dry season vs. wet season AOR 2.9; 95% CI 1.6-5.2). Conclusion: GBS colonisation is common in Gambian women at delivery and in their infants to day 60-89 and is dominated by serotype V. In addition to maternal colonisation, breastmilk and season of birth are important risk factors for infant GBS colonisation.

Original languageEnglish
Pages (from-to)283-294
Number of pages12
JournalJournal of Infection
Volume72
Issue number3
DOIs
Publication statusPublished - 1 Mar 2016

Bibliographical note

Funding Information:
This work was supported by a Wellcome Trust Clinical Research Fellowship to KLD ( WT104482MA ) and the Thrasher Research Fund (BK: 12250). BK is also supported by grants from the UK MRC ( MC_UP_A900/1122 , MC_UP_A900/115 ) and the UK Medical Research Council (MRC) and the Department for International Development (DFID) under the MRC/DFID Concordat arrangement.

Funding Information:
This work was supported by a Wellcome Trust Clinical Research Fellowship to KLD (WT104482MA) and the Thrasher Research Fund (BK: 12250). BK is also supported by grants from the UK MRC (MC_UP_A900/1122, MC_UP_A900/115) and the UK Medical Research Council (MRC) and the Department for International Development (DFID) under the MRC/DFID Concordat arrangement. The authors would like to thank the study participants and field workers at Faji Kunda and Jammeh Foundation for Peace Hospitals and the lab staff Amadou Faal, Francess Sarfo and Mustapha Jaiteh at MRC Unit The Gambia. We would like to thank Martin Antonio, Ebenezer Foster-Nyarko and Edward Clarke for their support at the MRC Unit The Gambia.

Publisher Copyright:
© 2015.

Keywords

  • Group B Streptococcus
  • Immunity
  • Neonatal infection
  • Vaccines

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