RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay)

Julie Depuydt, Ans Baeyens, Stephen Barnard, Christina Beinke, Anett Benedek, Philip Beukes, Iwona Buraczewska, Firouz Darroudi, Stefania De Sanctis, Inmaculada Dominguez, Octávia Monteiro Gil, Valeria Hadjidekova, Enikő Kis, Ulrike Kulka, Florigio Lista, Katalin Lumniczky, Radhia M’kacher, Jayne Moquet, Doina Obreja, Ursula OestreicherJelena Pajic, Nuria Pastor, Ljubomira Popova, Elisa Regalbuto, Michelle Ricoul, Laure Sabatier, Jacobus Slabbert, Sylwester Sommer, Antonella Testa, Hubert Thierens, Andrzej Wojcik, Anne Vral*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)


Purpose: In the framework of the ‘Realizing the European Network of Biodosimetry’ (RENEB) project, two intercomparison exercises were conducted to assess the suitability of an optimized version of the cytokinesis-block micronucleus assay, and to evaluate the capacity of a large laboratory network performing biodosimetry for radiation emergency triages. Twelve European institutions participated in the first exercise, and four non-RENEB labs were added in the second one. Materials and methods: Irradiated blood samples were shipped to participating labs, whose task was to culture these samples and provide a blind dose estimate. Micronucleus analysis was performed by automated, semi-automated and manual procedures. Results: The dose estimates provided by network laboratories were in good agreement with true administered doses. The most accurate estimates were reported for low dose points (≤ 0.94 Gy). For higher dose points (≥ 2.7 Gy) a larger variation in estimates was observed, though in the second exercise the number of acceptable estimates increased satisfactorily. Higher accuracy was achieved with the semi-automated method. Conclusion: The results of the two exercises performed by our network demonstrate that the micronucleus assay is a useful tool for large-scale radiation emergencies, and can be successfully implemented within a large network of laboratories.

Original languageEnglish
Pages (from-to)36-47
Number of pages12
JournalInternational Journal of Radiation Biology
Issue number1
Publication statusPublished - 2 Jan 2017

Bibliographical note

Funding Information:
We wish to thank Ms. Toke Thiron, Johanna Aernoudt, Greet De Smet and Leen Pieters for their excellent technical assistance. This work was supported by the EU within the 7th Framework Programme, grant number 295513 and by a University Development Cooperation ‘VLIR Own Initiative Programme’ between Belgium and South Africa (ZEIN2011PR387).

Publisher Copyright:
© 2016 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.


  • Biodosimetry
  • micronucleus assay
  • radiation accident
  • triage


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