RENEB biodosimetry intercomparison analyzing translocations by FISH

Joan Francesc Barquinero; Christina Beinke; Mireia Borràs; Iwona Buraczewska; Firouz Darroudi; Eric Gregoire; Rositsa Hristova; Ulrike Kulka; Carita Lindholm; Mercedes Moreno; Jayne Moquet; Ursula Oestreicher; M. Jesús Prieto; Mònica Pujol; Michelle Ricoul; Laure Sabatier; Sylwester Sommer; Mingzhu Sun; Andrzej Wojcik; Leonardo Barrios

doi:10.1080/09553002.2016.1222092

RENEB biodosimetry intercomparison analyzing translocations by FISH

Joan Francesc Barquinero, Christina Beinke, Mireia Borràs, Iwona Buraczewska, Firouz Darroudi, Eric Gregoire, Rositsa Hristova, Ulrike Kulka, Carita Lindholm, Mercedes Moreno, Jayne Moquet, Ursula Oestreicher, M. Jesús Prieto, Mònica Pujol, Michelle Ricoul, Laure Sabatier, Sylwester Sommer, Mingzhu Sun, Andrzej Wojcik, Leonardo Barrios^*

^*Corresponding author for this work

Radiation Effects

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Purpose: In the framework of RENEB, several biodosimetry exercises were conducted analyzing different endpoints. Among them, the analysis of translocations is considered the most useful method for retrospective biodosimetry due to the relative stability of their frequency with post irradiation time. The aim of this study was to harmonize the accuracy of translocation-based biodosimetry within the RENEB consortium. Materials and methods: An initial telescoring exercise analyzing FISH metaphase images was done to harmonize chromosome aberration descriptions. Then two blind intercomparison exercises (IE) were performed, by sending irradiated blood samples to each partner. Samples were cultured and stained by each partner using their standard protocol and translocation frequency was used to produce dose estimates. Results: The coefficient of variation in the 1st IE (CV = 0.34) was higher than in the 2nd IE (CV = 0.16 and 0.23 in the two samples analyzed), for the genomic frequency of total translocations. Z-score analysis revealed that eight out of 10 and 17 out of 20 dose estimates were satisfactory in the 1st and 2nd IE, respectively. Conclusions: The results obtained indicate that, despite the problems identified in few partners, which can be corrected, the RENEB consortium is able to carry out retrospective biodosimetry analyzing the frequency of translocations by FISH.

Original language	English
Pages (from-to)	30-35
Number of pages	6
Journal	International Journal of Radiation Biology
Volume	93
Issue number	1
DOIs	https://doi.org/10.1080/09553002.2016.1222092
Publication status	Published - 2 Jan 2017

Bibliographical note

Funding Information:
This work received financial support from the European Seventh Framework Programme (295513). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Publisher Copyright:
© 2016 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

Keywords

Biodosimetry
FISH
RENEB
translocations

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10.1080/09553002.2016.1222092

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Barquinero, J. F., Beinke, C., Borràs, M., Buraczewska, I., Darroudi, F., Gregoire, E., Hristova, R., Kulka, U., Lindholm, C., Moreno, M., Moquet, J., Oestreicher, U., Prieto, M. J., Pujol, M., Ricoul, M., Sabatier, L., Sommer, S., Sun, M., Wojcik, A., & Barrios, L. (2017). RENEB biodosimetry intercomparison analyzing translocations by FISH. International Journal of Radiation Biology, 93(1), 30-35. https://doi.org/10.1080/09553002.2016.1222092

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title = "RENEB biodosimetry intercomparison analyzing translocations by FISH",

abstract = "Purpose: In the framework of RENEB, several biodosimetry exercises were conducted analyzing different endpoints. Among them, the analysis of translocations is considered the most useful method for retrospective biodosimetry due to the relative stability of their frequency with post irradiation time. The aim of this study was to harmonize the accuracy of translocation-based biodosimetry within the RENEB consortium. Materials and methods: An initial telescoring exercise analyzing FISH metaphase images was done to harmonize chromosome aberration descriptions. Then two blind intercomparison exercises (IE) were performed, by sending irradiated blood samples to each partner. Samples were cultured and stained by each partner using their standard protocol and translocation frequency was used to produce dose estimates. Results: The coefficient of variation in the 1st IE (CV = 0.34) was higher than in the 2nd IE (CV = 0.16 and 0.23 in the two samples analyzed), for the genomic frequency of total translocations. Z-score analysis revealed that eight out of 10 and 17 out of 20 dose estimates were satisfactory in the 1st and 2nd IE, respectively. Conclusions: The results obtained indicate that, despite the problems identified in few partners, which can be corrected, the RENEB consortium is able to carry out retrospective biodosimetry analyzing the frequency of translocations by FISH.",

keywords = "Biodosimetry, FISH, RENEB, translocations",

author = "Barquinero, {Joan Francesc} and Christina Beinke and Mireia Borr{\`a}s and Iwona Buraczewska and Firouz Darroudi and Eric Gregoire and Rositsa Hristova and Ulrike Kulka and Carita Lindholm and Mercedes Moreno and Jayne Moquet and Ursula Oestreicher and Prieto, {M. Jes{\'u}s} and M{\`o}nica Pujol and Michelle Ricoul and Laure Sabatier and Sylwester Sommer and Mingzhu Sun and Andrzej Wojcik and Leonardo Barrios",

note = "Funding Information: This work received financial support from the European Seventh Framework Programme (295513). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: {\textcopyright} 2016 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.",

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Barquinero, JF, Beinke, C, Borràs, M, Buraczewska, I, Darroudi, F, Gregoire, E, Hristova, R, Kulka, U, Lindholm, C, Moreno, M, Moquet, J, Oestreicher, U, Prieto, MJ, Pujol, M, Ricoul, M, Sabatier, L, Sommer, S, Sun, M, Wojcik, A & Barrios, L 2017, 'RENEB biodosimetry intercomparison analyzing translocations by FISH', International Journal of Radiation Biology, vol. 93, no. 1, pp. 30-35. https://doi.org/10.1080/09553002.2016.1222092

TY - JOUR

T1 - RENEB biodosimetry intercomparison analyzing translocations by FISH

AU - Barquinero, Joan Francesc

AU - Beinke, Christina

AU - Borràs, Mireia

AU - Buraczewska, Iwona

AU - Darroudi, Firouz

AU - Gregoire, Eric

AU - Hristova, Rositsa

AU - Kulka, Ulrike

AU - Lindholm, Carita

AU - Moreno, Mercedes

AU - Moquet, Jayne

AU - Oestreicher, Ursula

AU - Prieto, M. Jesús

AU - Pujol, Mònica

AU - Ricoul, Michelle

AU - Sabatier, Laure

AU - Sommer, Sylwester

AU - Sun, Mingzhu

AU - Wojcik, Andrzej

AU - Barrios, Leonardo

N1 - Funding Information: This work received financial support from the European Seventh Framework Programme (295513). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: © 2016 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

PY - 2017/1/2

Y1 - 2017/1/2

N2 - Purpose: In the framework of RENEB, several biodosimetry exercises were conducted analyzing different endpoints. Among them, the analysis of translocations is considered the most useful method for retrospective biodosimetry due to the relative stability of their frequency with post irradiation time. The aim of this study was to harmonize the accuracy of translocation-based biodosimetry within the RENEB consortium. Materials and methods: An initial telescoring exercise analyzing FISH metaphase images was done to harmonize chromosome aberration descriptions. Then two blind intercomparison exercises (IE) were performed, by sending irradiated blood samples to each partner. Samples were cultured and stained by each partner using their standard protocol and translocation frequency was used to produce dose estimates. Results: The coefficient of variation in the 1st IE (CV = 0.34) was higher than in the 2nd IE (CV = 0.16 and 0.23 in the two samples analyzed), for the genomic frequency of total translocations. Z-score analysis revealed that eight out of 10 and 17 out of 20 dose estimates were satisfactory in the 1st and 2nd IE, respectively. Conclusions: The results obtained indicate that, despite the problems identified in few partners, which can be corrected, the RENEB consortium is able to carry out retrospective biodosimetry analyzing the frequency of translocations by FISH.

AB - Purpose: In the framework of RENEB, several biodosimetry exercises were conducted analyzing different endpoints. Among them, the analysis of translocations is considered the most useful method for retrospective biodosimetry due to the relative stability of their frequency with post irradiation time. The aim of this study was to harmonize the accuracy of translocation-based biodosimetry within the RENEB consortium. Materials and methods: An initial telescoring exercise analyzing FISH metaphase images was done to harmonize chromosome aberration descriptions. Then two blind intercomparison exercises (IE) were performed, by sending irradiated blood samples to each partner. Samples were cultured and stained by each partner using their standard protocol and translocation frequency was used to produce dose estimates. Results: The coefficient of variation in the 1st IE (CV = 0.34) was higher than in the 2nd IE (CV = 0.16 and 0.23 in the two samples analyzed), for the genomic frequency of total translocations. Z-score analysis revealed that eight out of 10 and 17 out of 20 dose estimates were satisfactory in the 1st and 2nd IE, respectively. Conclusions: The results obtained indicate that, despite the problems identified in few partners, which can be corrected, the RENEB consortium is able to carry out retrospective biodosimetry analyzing the frequency of translocations by FISH.

KW - Biodosimetry

KW - FISH

KW - RENEB

KW - translocations

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DO - 10.1080/09553002.2016.1222092

M3 - Article

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SN - 0955-3002

VL - 93

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EP - 35

JO - International Journal of Radiation Biology

JF - International Journal of Radiation Biology

IS - 1

ER -

RENEB biodosimetry intercomparison analyzing translocations by FISH

Abstract

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Keywords

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