Real-time TaqMan PCR for rapid detection and typing of genes encoding CTX-M extended-spectrum β-lactamases

Christopher I. Birkett*, Hugo A. Ludlam, Neil Woodford, Derek F.J. Brown, Nicholas M. Brown, Mark T.M. Roberts, Nicola Milner, Martin D. Curran

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    107 Citations (Scopus)

    Abstract

    The prevalence of CTX-M-producing members of the Enterobacteriaceae is increasing worldwide. A novel, multiplex, real-time TaqMan PCR assay to detect and type blaCTX-M genes is described which is an improvement on previously described techniques with respect to reduced assay time, elimination of the need for protracted post-PCR processing and the convenience of a single reaction vessel. Based on β-lactam antibiogram and MIC data, 478 of 1279 Enterobacteriaceae isolates from clinical blood and urine culture specimens were selected and tested for extended-spectrum β-lactamase (ESBL) production using phenotypic methods. The new TaqMan assay detected and typed bla CTX-M genes in 21 of 28 ESBL-producing isolates.

    Original languageEnglish
    Pages (from-to)52-55
    Number of pages4
    JournalJournal of Medical Microbiology
    Volume56
    Issue number1
    DOIs
    Publication statusPublished - Jan 2007

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