Real-time PCRs and fingerprinting assays for the detection and characterization of Salmonella genomic island-1 encoding multidrug resistance: Application to 445 European isolates of Salmonella, Escherichia coli, Shigella, and Proteus

Corinne Amar, Catherine Arnold, Alan Bankier, Paul H. Dear, Beatriz Guerra, Katie L. Hopkins, Ernesto Liebana, Dik J. Mevius, John Threlfall

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    18 Citations (Scopus)

    Abstract

    Salmonella Genomic Island-1 (SGI-1) harbors a cluster of genes encoding multidrug resistance (MDR). SGI-1 is horizontally transmissible and is therefore of significant public health concern. This study presents two novel realtime PCRs detecting three SGI-1 protein-coding genes and a SGI-1 fingerprinting assay. These assays were applied to 445 European enterobacterial isolates. Results from real-time PCRs were comparable to those obtained from gelbased PCRs used for the detection of SGI-1, but were rapid to perform and suitable for large-scale screening. Furthermore, real-time PCRs also detected SGI-1 even when only part of the island was present in bacterial isolates. No trace of SGI-1 was detected in isolates other than Salmonella enterica. The fingerprints showed that regions of SGI-1 outside the MDR region exhibited genomic variations between isolates. In conclusion, the realtime PCRs described here are suitable for the detection of SGI-1 in bacterial isolates. Further studies are necessary to elucidate divergence in its non-MDR region.

    Original languageEnglish
    Pages (from-to)79-92
    Number of pages14
    JournalMicrobial Drug Resistance
    Volume14
    Issue number2
    DOIs
    Publication statusPublished - 1 Jun 2008

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