Real-time PCR for detection of the O25b-ST131 clone of Escherichia coli and its CTX-M-15-like extended-spectrum β-lactamases

Hiran Dhanji; Michel Doumith; Olivier Clermont; Erick Denamur; Russell Hope; David M. Livermore; Neil Woodford

doi:10.1016/j.ijantimicag.2010.06.007

Real-time PCR for detection of the O25b-ST131 clone of Escherichia coli and its CTX-M-15-like extended-spectrum β-lactamases

Hiran Dhanji^*, Michel Doumith, Olivier Clermont, Erick Denamur, Russell Hope, David M. Livermore, Neil Woodford

^*Corresponding author for this work

Healthcare Associated Infections Surveillance General

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

CTX-M-15 has become the most prevalent extended-spectrum β-lactamase amongst Escherichia coli in many countries during the past decade. Its dominance partly reflects the dissemination of an E. coli O25b:H4 ST131 clone that commonly produces this enzyme. We describe rapid real-time polymerase chain reaction (PCR) assays able to detect E. coli belonging to the ST131 clone and to identify bla_CTX-M-15-like.

Original language	English
Pages (from-to)	355-358
Number of pages	4
Journal	International Journal of Antimicrobial Agents
Volume	36
Issue number	4
DOIs	https://doi.org/10.1016/j.ijantimicag.2010.06.007
Publication status	Published - Oct 2010

Keywords

ESBL
Epidemic
Escherichia coli
LightCycler PCR
Pandemic clone

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ijantimicag.2010.06.007

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title = "Real-time PCR for detection of the O25b-ST131 clone of Escherichia coli and its CTX-M-15-like extended-spectrum β-lactamases",

abstract = "CTX-M-15 has become the most prevalent extended-spectrum β-lactamase amongst Escherichia coli in many countries during the past decade. Its dominance partly reflects the dissemination of an E. coli O25b:H4 ST131 clone that commonly produces this enzyme. We describe rapid real-time polymerase chain reaction (PCR) assays able to detect E. coli belonging to the ST131 clone and to identify blaCTX-M-15-like.",

keywords = "ESBL, Epidemic, Escherichia coli, LightCycler PCR, Pandemic clone",

author = "Hiran Dhanji and Michel Doumith and Olivier Clermont and Erick Denamur and Russell Hope and Livermore, {David M.} and Neil Woodford",

year = "2010",

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language = "English",

volume = "36",

pages = "355--358",

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T1 - Real-time PCR for detection of the O25b-ST131 clone of Escherichia coli and its CTX-M-15-like extended-spectrum β-lactamases

AU - Dhanji, Hiran

AU - Doumith, Michel

AU - Clermont, Olivier

AU - Denamur, Erick

AU - Hope, Russell

AU - Livermore, David M.

AU - Woodford, Neil

PY - 2010/10

Y1 - 2010/10

N2 - CTX-M-15 has become the most prevalent extended-spectrum β-lactamase amongst Escherichia coli in many countries during the past decade. Its dominance partly reflects the dissemination of an E. coli O25b:H4 ST131 clone that commonly produces this enzyme. We describe rapid real-time polymerase chain reaction (PCR) assays able to detect E. coli belonging to the ST131 clone and to identify blaCTX-M-15-like.

AB - CTX-M-15 has become the most prevalent extended-spectrum β-lactamase amongst Escherichia coli in many countries during the past decade. Its dominance partly reflects the dissemination of an E. coli O25b:H4 ST131 clone that commonly produces this enzyme. We describe rapid real-time polymerase chain reaction (PCR) assays able to detect E. coli belonging to the ST131 clone and to identify blaCTX-M-15-like.

KW - ESBL

KW - Epidemic

KW - Escherichia coli

KW - LightCycler PCR

KW - Pandemic clone

UR - http://www.scopus.com/inward/record.url?scp=77955924829&partnerID=8YFLogxK

U2 - 10.1016/j.ijantimicag.2010.06.007

DO - 10.1016/j.ijantimicag.2010.06.007

M3 - Article

C2 - 20691573

AN - SCOPUS:77955924829

SN - 0924-8579

VL - 36

SP - 355

EP - 358

JO - International Journal of Antimicrobial Agents

JF - International Journal of Antimicrobial Agents

IS - 4

ER -

Real-time PCR for detection of the O25b-ST131 clone of Escherichia coli and its CTX-M-15-like extended-spectrum β-lactamases

Abstract

Keywords

UN SDGs

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