Real-time PCR assay for differentiation of typhoidal and nontyphoidal Salmonella

Satheesh Nair; Vineet Patel; Tadgh Hickey; Clare Maguire; David Greig; Winnie Lee; Gauri Godbole; Kathleen Grant; Marie Anne Chattaway

doi:10.1128/JCM.00167-19

Real-time PCR assay for differentiation of typhoidal and nontyphoidal Salmonella

Satheesh Nair^*, Vineet Patel, Tadgh Hickey, Clare Maguire, David Greig, Winnie Lee, Gauri Godbole, Kathleen Grant, Marie Anne Chattaway

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Rapid and accurate differentiation of Salmonella spp. causing enteric fever from nontyphoidal Salmonella is essential for clinical management of cases, laboratory risk management, and implementation of public health measures. Current methods used for confirmation of identification, including biochemistry and serotyping as well as whole-genome sequencing analyses, take several days. Here we report the development and evaluation of a real-time PCR assay that can be performed directly on crude DNA extracts from bacterial colonies for the rapid identification of typhoidal and nontyphoidal Salmonella.

Original language	English
Article number	e00167-19
Journal	Journal of Clinical Microbiology
Volume	57
Issue number	8
DOIs	https://doi.org/10.1128/JCM.00167-19
Publication status	Published - 2019

Bibliographical note

Funding Information:
We thank Sarah Alexandra and Julie Russell from the National Collection of Type Cultures for providing positive-control strains. We also thank Lailanie Aqunino from GBRU for support in undertaking PCR. We have no conflict of interest to declare. This study was funded by PHE.

Publisher Copyright:
© Crown copyright 2019.

Keywords

Nontyphoidal salmonellae
Real-time PCR
Salmonella
Typhoidal salmonellae
Whole-genome sequencing

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/JCM.00167-19

Cite this

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abstract = "Rapid and accurate differentiation of Salmonella spp. causing enteric fever from nontyphoidal Salmonella is essential for clinical management of cases, laboratory risk management, and implementation of public health measures. Current methods used for confirmation of identification, including biochemistry and serotyping as well as whole-genome sequencing analyses, take several days. Here we report the development and evaluation of a real-time PCR assay that can be performed directly on crude DNA extracts from bacterial colonies for the rapid identification of typhoidal and nontyphoidal Salmonella.",

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author = "Satheesh Nair and Vineet Patel and Tadgh Hickey and Clare Maguire and David Greig and Winnie Lee and Gauri Godbole and Kathleen Grant and Chattaway, {Marie Anne}",

note = "Funding Information: We thank Sarah Alexandra and Julie Russell from the National Collection of Type Cultures for providing positive-control strains. We also thank Lailanie Aqunino from GBRU for support in undertaking PCR. We have no conflict of interest to declare. This study was funded by PHE. Publisher Copyright: {\textcopyright} Crown copyright 2019.",

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AU - Nair, Satheesh

AU - Patel, Vineet

AU - Hickey, Tadgh

AU - Maguire, Clare

AU - Greig, David

AU - Lee, Winnie

AU - Godbole, Gauri

AU - Grant, Kathleen

AU - Chattaway, Marie Anne

N1 - Funding Information: We thank Sarah Alexandra and Julie Russell from the National Collection of Type Cultures for providing positive-control strains. We also thank Lailanie Aqunino from GBRU for support in undertaking PCR. We have no conflict of interest to declare. This study was funded by PHE. Publisher Copyright: © Crown copyright 2019.

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Y1 - 2019

N2 - Rapid and accurate differentiation of Salmonella spp. causing enteric fever from nontyphoidal Salmonella is essential for clinical management of cases, laboratory risk management, and implementation of public health measures. Current methods used for confirmation of identification, including biochemistry and serotyping as well as whole-genome sequencing analyses, take several days. Here we report the development and evaluation of a real-time PCR assay that can be performed directly on crude DNA extracts from bacterial colonies for the rapid identification of typhoidal and nontyphoidal Salmonella.

AB - Rapid and accurate differentiation of Salmonella spp. causing enteric fever from nontyphoidal Salmonella is essential for clinical management of cases, laboratory risk management, and implementation of public health measures. Current methods used for confirmation of identification, including biochemistry and serotyping as well as whole-genome sequencing analyses, take several days. Here we report the development and evaluation of a real-time PCR assay that can be performed directly on crude DNA extracts from bacterial colonies for the rapid identification of typhoidal and nontyphoidal Salmonella.

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KW - Typhoidal salmonellae

KW - Whole-genome sequencing

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Real-time PCR assay for differentiation of typhoidal and nontyphoidal Salmonella

Abstract

Bibliographical note

Keywords

UN SDGs

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