Reaction-based indicator displacement assay (RIA) for the development of a triggered release system capable of biofilm inhibition

Bethany L. Patenall, George T. Williams‡, Lauren Gwynne, Liam J. Stephens, Emma V. Lampard, Hollie J. Hathaway, Naing T. Thet, Amber E. Young, Mark J. Sutton, Robert D. Short, Steven D. Bull, Tony D. James, Adam C. Sedgwick, A. Toby A. Jenkins*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Here, a reaction-based indicator displacement hydrogel assay (RIA) was developed for the detection of hydrogen peroxide (H2O2) via the oxidative release of the optical reporter Alizarin Red S (ARS). In the presence of H2O2, the RIA system displayed potent biofilm inhibition for Methicillin-resistant Staphylococcus aureus (MRSA), as shown through an in vitro assay quantifying antimicrobial efficacy. This work demonstrated the potential of H2O2-responsive hydrogels containing a covalently bound diol-based drug for controlled drug release.

Original languageEnglish
Pages (from-to)15129-15132
Number of pages4
JournalChemical Communications
Volume55
Issue number100
DOIs
Publication statusPublished - 2019

Bibliographical note

Publisher Copyright:
© 2019 The Royal Society of Chemistry.

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