Reaction-based indicator displacement assay (RIA) for the development of a triggered release system capable of biofilm inhibition

Bethany L. Patenall, George T. Williams‡, Lauren Gwynne, Liam J. Stephens, Emma V. Lampard, Hollie J. Hathaway, Naing T. Thet, Amber E. Young, Mark J. Sutton, Robert D. Short, Steven D. Bull, Tony D. James, Adam C. Sedgwick, A. Toby A. Jenkins*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Here, a reaction-based indicator displacement hydrogel assay (RIA) was developed for the detection of hydrogen peroxide (H2O2) via the oxidative release of the optical reporter Alizarin Red S (ARS). In the presence of H2O2, the RIA system displayed potent biofilm inhibition for Methicillin-resistant Staphylococcus aureus (MRSA), as shown through an in vitro assay quantifying antimicrobial efficacy. This work demonstrated the potential of H2O2-responsive hydrogels containing a covalently bound diol-based drug for controlled drug release.

Original languageEnglish
Pages (from-to)15129-15132
Number of pages4
JournalChemical Communications
Volume55
Issue number100
DOIs
Publication statusPublished - 2019

Bibliographical note

Funding Information:
The authors would like to thank the EPSRC for grant EP/ R003556/1. BLP would also like thank James Tudor and Mr and Mrs Watson for additional funding. GTW would like to thank the EPSRC and Public Health England. ACS, ATAJ and NTT wish to thank the EPSRC for funding on Smartwound plasma – EP/R003939/1. TDJ wishes to thank the Royal Society for a Wolfson Research Merit Award. We would like to thank James T. Brewster II at Harvard college for helpful discussions and suggestions to improve the manuscript.

Publisher Copyright:
© 2019 The Royal Society of Chemistry.

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