Rapid methods for the extraction and archiving of molecular grade fungal genomic DNA

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    3 Citations (Scopus)

    Abstract

    The rapid and inexpensive extraction of fungal genomic DNA that is of suf ficient quality for molecular approaches is central to the molecular identification, epidemiological analysis, taxonomy, and strain typing of pathogenic fungi. Although many commercially available and in-house extraction procedures do eliminate the majority of contaminants that commonly inhibit molecular approaches, the inherent difficulties in breaking fungal cell walls lead to protocols that are labor intensive and that routinely take several hours to complete. Here we describe several methods that we have developed in our laboratory that allow the extremely rapid and inexpensive preparation of fungal genomic DNA.

    Original languageEnglish
    Title of host publicationFungal Diagnostics
    Subtitle of host publicationMethods and Protocols
    PublisherHumana Press Inc.
    Pages55-62
    Number of pages8
    ISBN (Print)9781627032568
    DOIs
    Publication statusPublished - 2013

    Publication series

    NameMethods in Molecular Biology
    Volume968
    ISSN (Print)1064-3745

    Keywords

    • FTA matrix
    • Fungi
    • Genomic DNA
    • Mold
    • PCR
    • Yeast

    Fingerprint

    Dive into the research topics of 'Rapid methods for the extraction and archiving of molecular grade fungal genomic DNA'. Together they form a unique fingerprint.

    Cite this