Rapid genomic typing of BK virus directly from clinical specimens

Li Jin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

A simple and rapid method, PCR-restriction enzyme analysis (PCR-RE) for BK virus (BKV) typing was developed, based on the presence of type-specific restriction enzyme sites in a 327 bp PCR-generated fragment which partially encodes the VP1 protein. The enzymes, Alu I, Xmn I and Ava II, were used to digest the PCR products in two stages. Ethidium bromide banding patterns characteristic of each of four subtypes of BKV were visualized through gel electrophoresis. A total of 37 samples from clinical specimens and culture fluids were successfully subtyped using the PCR-RE. A second method, PCR-sequencing, was applied to samples that generated less than 100 ng of DNA. These were subjected to a second round of PCR and then sequenced from single stranded templates immobilised via a biotinylated primer. The subtypes were assigned on the basis of the type-specific sequences previously characterized.

Original languageEnglish
Pages (from-to)331-334
Number of pages4
JournalMolecular and Cellular Probes
Volume7
Issue number4
DOIs
Publication statusPublished - 30 Aug 1993
Externally publishedYes

Keywords

  • BK virus
  • Genomic subtypes
  • PCR-restriction enzyme analysis

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