Rapid detection of the O25b-ST131 clone of Escherichia coli encompassing the CTX-M-15-producing strains

Olivier Clermont; Hiran Dhanji; Mathew Upton; Tarek Gibreel; Andrew Fox; David Boyd; Michael R. Mulvey; Patrice Nordmann; Etienne Ruppé; Jean Louis Sarthou; Thierry Frank; Sophie Vimont; Guillaume Arlet; Catherine Branger; Neil Woodford; Erick Denamur

doi:10.1093/jac/dkp194

Rapid detection of the O25b-ST131 clone of Escherichia coli encompassing the CTX-M-15-producing strains

Olivier Clermont, Hiran Dhanji, Mathew Upton, Tarek Gibreel, Andrew Fox, David Boyd, Michael R. Mulvey, Patrice Nordmann, Etienne Ruppé, Jean Louis Sarthou, Thierry Frank, Sophie Vimont, Guillaume Arlet, Catherine Branger, Neil Woodford, Erick Denamur^*

^*Corresponding author for this work

Field Service Divisional Office

Research output: Contribution to journal › Article › peer-review

319 Citations (Scopus)

Abstract

Objectives: Recently, a CTX-M-15 extended-spectrum β-lactamase (ESBL)-producing Escherichia coli O25b-ST131 clone, belonging to the B2 phylogenetic group and with a high virulence potential, has been reported all over the world, representing a major public health problem. The present study was carried out to develop a rapid and simple detection assay that identifies members of this clone. Methods: A totalof 627 E. coli isolates of which 373 produced an ESBL, collected across four continents, were screened using a O25b-ST131 clone allele-specific PCR for the pabB gene. Results: One hundred and forty-three ESBL isolates were found positive with the assay. These isolates were all of O25b type and, when studied by multilocus sequence typing (25 cases), were all of ST131. The O25b-ST131 clone was found to produce ESBLs other than CTX-M-15, specifically CTX-M-2, -3, -14, -27, -32 and -61 as well as TEM-24. This clone represents 3% of non-ESBL B2 isolates originating from urinary tract infections in Paris. Conclusions: We have developed a PCR-based assay that easily identifies a clone with high likelihood of producing ESBLs, including CTX-M-15.

Original language	English
Pages (from-to)	274-277
Number of pages	4
Journal	Journal of Antimicrobial Chemotherapy
Volume	64
Issue number	2
DOIs	https://doi.org/10.1093/jac/dkp194
Publication status	Published - 2009

Bibliographical note

Funding Information:
This work was partially funded by a ‘Contrat d’Initiation à la Recherche Clinique’ CRC 05103 (Assistance Publique, Hôpitaux de Paris) and the ‘Fondation pour la Recherche Médicale’.

Keywords

B2 phylogenetic subgroup I
ESBL
O25
ST 131

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1093/jac/dkp194

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Clermont, O., Dhanji, H., Upton, M., Gibreel, T., Fox, A., Boyd, D., Mulvey, M. R., Nordmann, P., Ruppé, E., Sarthou, J. L., Frank, T., Vimont, S., Arlet, G., Branger, C., Woodford, N., & Denamur, E. (2009). Rapid detection of the O25b-ST131 clone of Escherichia coli encompassing the CTX-M-15-producing strains. Journal of Antimicrobial Chemotherapy, 64(2), 274-277. https://doi.org/10.1093/jac/dkp194

@article{a365132c97004ef492c94b86c203c40b,

title = "Rapid detection of the O25b-ST131 clone of Escherichia coli encompassing the CTX-M-15-producing strains",

abstract = "Objectives: Recently, a CTX-M-15 extended-spectrum β-lactamase (ESBL)-producing Escherichia coli O25b-ST131 clone, belonging to the B2 phylogenetic group and with a high virulence potential, has been reported all over the world, representing a major public health problem. The present study was carried out to develop a rapid and simple detection assay that identifies members of this clone. Methods: A totalof 627 E. coli isolates of which 373 produced an ESBL, collected across four continents, were screened using a O25b-ST131 clone allele-specific PCR for the pabB gene. Results: One hundred and forty-three ESBL isolates were found positive with the assay. These isolates were all of O25b type and, when studied by multilocus sequence typing (25 cases), were all of ST131. The O25b-ST131 clone was found to produce ESBLs other than CTX-M-15, specifically CTX-M-2, -3, -14, -27, -32 and -61 as well as TEM-24. This clone represents 3% of non-ESBL B2 isolates originating from urinary tract infections in Paris. Conclusions: We have developed a PCR-based assay that easily identifies a clone with high likelihood of producing ESBLs, including CTX-M-15.",

keywords = "B2 phylogenetic subgroup I, ESBL, O25, ST 131",

author = "Olivier Clermont and Hiran Dhanji and Mathew Upton and Tarek Gibreel and Andrew Fox and David Boyd and Mulvey, {Michael R.} and Patrice Nordmann and Etienne Rupp{\'e} and Sarthou, {Jean Louis} and Thierry Frank and Sophie Vimont and Guillaume Arlet and Catherine Branger and Neil Woodford and Erick Denamur",

note = "Funding Information: This work was partially funded by a {\textquoteleft}Contrat d{\textquoteright}Initiation {\`a} la Recherche Clinique{\textquoteright} CRC 05103 (Assistance Publique, H{\^o}pitaux de Paris) and the {\textquoteleft}Fondation pour la Recherche M{\'e}dicale{\textquoteright}.",

year = "2009",

doi = "10.1093/jac/dkp194",

language = "English",

volume = "64",

pages = "274--277",

journal = "Journal of Antimicrobial Chemotherapy",

issn = "0305-7453",

publisher = "Oxford University Press",

number = "2",

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Clermont, O, Dhanji, H, Upton, M, Gibreel, T, Fox, A, Boyd, D, Mulvey, MR, Nordmann, P, Ruppé, E, Sarthou, JL, Frank, T, Vimont, S, Arlet, G, Branger, C, Woodford, N & Denamur, E 2009, 'Rapid detection of the O25b-ST131 clone of Escherichia coli encompassing the CTX-M-15-producing strains', Journal of Antimicrobial Chemotherapy, vol. 64, no. 2, pp. 274-277. https://doi.org/10.1093/jac/dkp194

TY - JOUR

T1 - Rapid detection of the O25b-ST131 clone of Escherichia coli encompassing the CTX-M-15-producing strains

AU - Clermont, Olivier

AU - Dhanji, Hiran

AU - Upton, Mathew

AU - Gibreel, Tarek

AU - Fox, Andrew

AU - Boyd, David

AU - Mulvey, Michael R.

AU - Nordmann, Patrice

AU - Ruppé, Etienne

AU - Sarthou, Jean Louis

AU - Frank, Thierry

AU - Vimont, Sophie

AU - Arlet, Guillaume

AU - Branger, Catherine

AU - Woodford, Neil

AU - Denamur, Erick

N1 - Funding Information: This work was partially funded by a ‘Contrat d’Initiation à la Recherche Clinique’ CRC 05103 (Assistance Publique, Hôpitaux de Paris) and the ‘Fondation pour la Recherche Médicale’.

PY - 2009

Y1 - 2009

N2 - Objectives: Recently, a CTX-M-15 extended-spectrum β-lactamase (ESBL)-producing Escherichia coli O25b-ST131 clone, belonging to the B2 phylogenetic group and with a high virulence potential, has been reported all over the world, representing a major public health problem. The present study was carried out to develop a rapid and simple detection assay that identifies members of this clone. Methods: A totalof 627 E. coli isolates of which 373 produced an ESBL, collected across four continents, were screened using a O25b-ST131 clone allele-specific PCR for the pabB gene. Results: One hundred and forty-three ESBL isolates were found positive with the assay. These isolates were all of O25b type and, when studied by multilocus sequence typing (25 cases), were all of ST131. The O25b-ST131 clone was found to produce ESBLs other than CTX-M-15, specifically CTX-M-2, -3, -14, -27, -32 and -61 as well as TEM-24. This clone represents 3% of non-ESBL B2 isolates originating from urinary tract infections in Paris. Conclusions: We have developed a PCR-based assay that easily identifies a clone with high likelihood of producing ESBLs, including CTX-M-15.

AB - Objectives: Recently, a CTX-M-15 extended-spectrum β-lactamase (ESBL)-producing Escherichia coli O25b-ST131 clone, belonging to the B2 phylogenetic group and with a high virulence potential, has been reported all over the world, representing a major public health problem. The present study was carried out to develop a rapid and simple detection assay that identifies members of this clone. Methods: A totalof 627 E. coli isolates of which 373 produced an ESBL, collected across four continents, were screened using a O25b-ST131 clone allele-specific PCR for the pabB gene. Results: One hundred and forty-three ESBL isolates were found positive with the assay. These isolates were all of O25b type and, when studied by multilocus sequence typing (25 cases), were all of ST131. The O25b-ST131 clone was found to produce ESBLs other than CTX-M-15, specifically CTX-M-2, -3, -14, -27, -32 and -61 as well as TEM-24. This clone represents 3% of non-ESBL B2 isolates originating from urinary tract infections in Paris. Conclusions: We have developed a PCR-based assay that easily identifies a clone with high likelihood of producing ESBLs, including CTX-M-15.

KW - B2 phylogenetic subgroup I

KW - ESBL

KW - O25

KW - ST 131

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SN - 0305-7453

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JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

IS - 2

ER -

Rapid detection of the O25b-ST131 clone of Escherichia coli encompassing the CTX-M-15-producing strains

Abstract

Bibliographical note

Keywords

UN SDGs

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