Rapid and simultaneous detection of genes encoding klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-β-lactamase (blaNDM) in gram-negative bacilli

Scott A. Cunningham, Tabassum Noorie, Daniele Meunier, Neil Woodford, Robin Patel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

Wepresent a duplex, real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-=- lactamase (blaNDM) genes. Accuracy was assessed with 158 Gram-negative bacillary isolates, including 134 carbapenemase producers. The assay had 100% sensitivity and specificity compared with reference methods and a turnaround time of 90 min.

Original languageEnglish
Pages (from-to)1269-1271
Number of pages3
JournalJournal of Clinical Microbiology
Volume51
Issue number4
DOIs
Publication statusPublished - Apr 2013

Bibliographical note

Funding Information:
a Grupo de Trabajo del Sistema Nervioso Periférico, Sociedad Neurológica, Ciudad Autónoma de Buenos Aires (CABA), Argentina b Unidad Asistencial por más Salud Dr. César Milstein, CABA, Argentina c Fundación para la Investigación en Neuroepidemiología(FINEP), Junín,Argentina d Instituto de Investigaciones Neurológicas Dr. Raúl Correa, Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia (FLENI), CABA, Argentina

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