TY - JOUR
T1 - Rapid analysis of resistant mutant genotypes using pyrosequencing.
AU - Arnold, Catherine
N1 - Copyright:
This record is sourced from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
PY - 2010
Y1 - 2010
N2 - The rapid detection of nucleotide mutations conferring drug resistance is especially important for organisms with long generation times. Mycobacterium tuberculosis, an organism thought to infect around one-third of the global population, is probably the most important of these slow-growers. Multidrug-resistant (MDR) strains of M. tuberculosis, indicated by resistance to rifampicin, are emerging and their rapid detection is crucial for treatment and control of this pathogen. Single nucleotide polymorphisms (SNPs) in the rifampicin resistance determining region (RRDR) of rpoB, an 81-bp region of the RNA polymerase B gene, confer resistance to rifampicin in the vast majority of cases. Combining PCR of this region and rapid short read sequencing using Pyrosequencing enables rapid high-throughput MDR strains to be detected from sputum and early cultures.
AB - The rapid detection of nucleotide mutations conferring drug resistance is especially important for organisms with long generation times. Mycobacterium tuberculosis, an organism thought to infect around one-third of the global population, is probably the most important of these slow-growers. Multidrug-resistant (MDR) strains of M. tuberculosis, indicated by resistance to rifampicin, are emerging and their rapid detection is crucial for treatment and control of this pathogen. Single nucleotide polymorphisms (SNPs) in the rifampicin resistance determining region (RRDR) of rpoB, an 81-bp region of the RNA polymerase B gene, confer resistance to rifampicin in the vast majority of cases. Combining PCR of this region and rapid short read sequencing using Pyrosequencing enables rapid high-throughput MDR strains to be detected from sputum and early cultures.
UR - http://www.scopus.com/inward/record.url?scp=77954666354&partnerID=8YFLogxK
U2 - 10.1007/978-1-60327-279-7_17
DO - 10.1007/978-1-60327-279-7_17
M3 - Article
C2 - 20401598
AN - SCOPUS:77954666354
SN - 1064-3745
VL - 642
SP - 217
EP - 223
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -
