Quantifying neutralising antibody responses against SARS-CoV-2 in dried blood spots (DBS) and paired sera

Kelly J. Roper, Jordan Thomas, Wejdan Albalawi, Emily Maddocks, Susan Dobson, Abdullateef Alshehri, Francesco G. Barone, Murielle Baltazar, Malcolm G. Semple, Antonia Ho, Lance Turtle, Cara Donegan, Rebecca G. Spencer, Shona C. Moore, Hayley Hardwick, Tom Solomon, Ana da Silva Filipe, Antonia Ying Wai Ho, Sarah E. McDonald, Massimo PalmariniDavid L. Robertson, Janet T. Scott, Emma C. Thomson, Meera Chand, Richard S. Tedder, Nicholas Price, Gary Leeming, Debby Bogaert, Clark D. Russell, Annemarie B. Docherty, Thomas M. Drake, Cameron J. Fairfield, Ewen M. Harrison, Stephen R. Knight, Kenneth A. Mclean, Derek Murphy, Lisa Norman, Riinu Pius, Catherine A. Shaw, Peter W. Horby, Olivia V. Swann, Kanta Chechi, A. A.Roger Thompson, Graham S. Cooke, Shiranee Sriskandan, Charlotte Summers, William Greenhalf, Vanessa Sancho-Shimizu, Saye Khoo, Mahdad Noursadeghi, David Stuart, Lucy Norris, Rishi K. Gupta, Andrew Rambaut, Christoper A. Green, Victoria Shaw, Seán Keating, Gail Carson, Laura Merson, Louise Sigfrid, James Lee, Daniel Plotkin, Marie Connor, Jo Dalton, Chloe Donohue, Carrol Gamble, Michelle Girvan, Sophie Halpin, Janet Harrison, Clare Jackson, Laura Marsh, Stephanie Roberts, Egle Saviciute, Tom Fletcher, Carlo Palmieri, Alison M. Meynert, Murray Wham, Peter J.M. Openshaw, Ryan S. Thwaites, Jake Dunning, Maria Zambon, Gonçalo dos Santos Correia, Matthew R. Lewis, Lynn Maslen, Caroline J. Sands, Panteleimon Takis, Wei Shen Lim, Alexander J. Mentzer, Paul Klenerman, Tassos Grammatikopoulos, Susan Knight, Sarah Tait, J. Kenneth Baillie, Sara Clohisey, Fiona Griffiths, Ross Hendry, Andrew Law, Wilna Oosthuyzen, Beatrice Alex, Benjamin Bach, James Scott-Brown, Petros Andrikopoulos, Marc Emmanuel Dumas, Julian L. Griffin, Sonia Liggi, Michael Olanipekun, Anthonia Osagie, Zoltan Takats, Wendy S. Barclay, Thushan de Silva, Samreen Ijaz, William A. Paxton, Georgios Pollakis*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


The ongoing SARS-CoV-2 pandemic was initially managed by non-pharmaceutical interventions such as diagnostic testing, isolation of positive cases, physical distancing and lockdowns. The advent of vaccines has provided crucial protection against SARS-CoV-2. Neutralising antibody (nAb) responses are a key correlate of protection, and therefore measuring nAb responses is essential for monitoring vaccine efficacy. Fingerstick dried blood spots (DBS) are ideal for use in large-scale sero-surveillance because they are inexpensive, offer the option of self-collection and can be transported and stored at ambient temperatures. Such advantages also make DBS appealing to use in resource-limited settings and in potential future pandemics. In this study, nAb responses in sera, venous blood and fingerstick blood stored on filter paper were measured. Samples were collected from SARS-CoV-2 acutely infected individuals, SARS-CoV-2 convalescent individuals and SARS-CoV-2 vaccinated individuals. Good agreement was observed between the nAb responses measured in eluted DBS and paired sera. Stability of nAb responses was also observed in sera stored on filter paper at room temperature for 28 days. Overall, this study provides support for the use of filter paper as a viable sample collection method to study nAb responses.

Original languageEnglish
Article number15014
JournalScientific Reports
Issue number1
Publication statusPublished - Dec 2023

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