Quantification of global transcription patterns in prokaryotes using spotted microarrays

Ben Sidders, Mike Withers, Sharon L. Kendall, Joanna Bacon, Simon J. Waddell, Jason Hinds, Paul Golby, Farahnaz Movahedzadeh, Robert A. Cox, Rosangela Frita, Annemieke M.C. ten Bokum, Lorenz Wernisch, Neil G. Stoker*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    36 Citations (Scopus)


    We describe an analysis, applicable to any spotted microarray dataset produced using genomic DNA as a reference, that quantifies prokaryotic levels of mRNA on a genome-wide scale. Applying this to Mycobacterium tuberculosis, we validate the technique, show a correlation between level of expression and biological importance, define the complement of invariant genes and analyze absolute levels of expression by functional class to develop ways of understanding an organism's biology without comparison to another growth condition.

    Original languageEnglish
    Article numberR265
    JournalGenome Biology
    Issue number12
    Publication statusPublished - 13 Dec 2007


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